Literature DB >> 22825488

Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag.

Evangelos Gatzogiannis1, Zhixing Chen, Lu Wei, Richard Wombacher, Ya-Ting Kao, Grygorii Yefremov, Virginia W Cornish, Wei Min.   

Abstract

The micro-viscosity and molecular crowding experienced by specific proteins can regulate their dynamics and function within live cells. Taking advantage of the emerging TMP-tag technology, we present the design, synthesis and application of a hybrid genetic-chemical molecular rotor probe whose fluorescence lifetime can report protein-specific micro-environments in live cells.

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Year:  2012        PMID： 22825488      PMCID： PMC3775488          DOI： 10.1039/c2cc33133k

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  29 in total

1.  Live-cell super-resolution imaging with trimethoprim conjugates.

Authors:  Richard Wombacher; Meike Heidbreder; Sebastian van de Linde; Michael P Sheetz; Mike Heilemann; Virginia W Cornish; Markus Sauer
Journal:  Nat Methods       Date:  2010-08-08       Impact factor: 28.547

Review 2.  Fluorescence lifetime measurements and biological imaging.

Authors:  Mikhail Y Berezin; Samuel Achilefu
Journal:  Chem Rev       Date:  2010-05-12       Impact factor: 60.622

Review 3.  Subnuclear organelles: new insights into form and function.

Authors:  Korie E Handwerger; Joseph G Gall
Journal:  Trends Cell Biol       Date:  2005-12-01       Impact factor: 20.808

4.  Chromatin structure exhibits spatio-temporal heterogeneity within the cell nucleus.

Authors:  Bidisha Banerjee; Dipanjan Bhattacharya; G V Shivashankar
Journal:  Biophys J       Date:  2006-06-30       Impact factor: 4.033

5.  Noninvasive determination of cell nucleoplasmic viscosity by fluorescence correlation spectroscopy.

Authors:  Lifang Liang; Xichao Wang; Da Xing; Tongsheng Chen; Wei R Chen
Journal:  J Biomed Opt       Date:  2009 Mar-Apr       Impact factor: 3.170

6.  Molecular rotor measures viscosity of live cells via fluorescence lifetime imaging.

Authors:  Marina K Kuimova; Gokhan Yahioglu; James A Levitt; Klaus Suhling
Journal:  J Am Chem Soc       Date:  2008-05-06       Impact factor: 15.419

7.  Fluorescence properties and photophysics of the sulfoindocyanine Cy3 linked covalently to DNA.

Authors:  Matthew E Sanborn; Brian K Connolly; Kaushik Gurunathan; Marcia Levitus
Journal:  J Phys Chem B       Date:  2007-08-24       Impact factor: 2.991

8.  Probing intranuclear environments at the single-molecule level.

Authors:  David Grünwald; Robert M Martin; Volker Buschmann; David P Bazett-Jones; Heinrich Leonhardt; Ulrich Kubitscheck; M Cristina Cardoso
Journal:  Biophys J       Date:  2007-12-07       Impact factor: 4.033

9.  Photophysics of Cy3-encapsulated calcium phosphate nanoparticles.

Authors:  Hari S Muddana; Thomas T Morgan; James H Adair; Peter J Butler
Journal:  Nano Lett       Date:  2009-04       Impact factor: 11.189

10.  Cytosolic viral sensor RIG-I is a 5'-triphosphate-dependent translocase on double-stranded RNA.

Authors:  Sua Myong; Sheng Cui; Peter V Cornish; Axel Kirchhofer; Michaela U Gack; Jae U Jung; Karl-Peter Hopfner; Taekjip Ha
Journal:  Science       Date:  2009-01-01       Impact factor: 47.728
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  6 in total

1.  Cytoplasmic delivery and selective, multicomponent labeling with oligoarginine-linked protein tags.

Authors:  Xiaoyan Zou; Megha Rajendran; Darren Magda; Lawrence W Miller
Journal:  Bioconjug Chem       Date:  2015-02-24       Impact factor: 4.774

2.  A Molecular Rotor that Measures Dynamic Changes of Lipid Bilayer Viscosity Caused by Oxidative Stress.

Authors:  Aurimas Vyšniauskas; Maryam Qurashi; Marina K Kuimova
Journal:  Chemistry       Date:  2016-08-03       Impact factor: 5.236

3.  Förster resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers.

Authors:  Evelyn Ploetz; Eitan Lerner; Florence Husada; Martin Roelfs; SangYoon Chung; Johannes Hohlbein; Shimon Weiss; Thorben Cordes
Journal:  Sci Rep       Date:  2016-09-19       Impact factor: 4.379

4.  Imaging tumor microscopic viscosity in vivo using molecular rotors.

Authors:  Lyubov' E Shimolina; Maria Angeles Izquierdo; Ismael López-Duarte; James A Bull; Marina V Shirmanova; Larisa G Klapshina; Elena V Zagaynova; Marina K Kuimova
Journal:  Sci Rep       Date:  2017-01-30       Impact factor: 4.379

5.  Tuning the Sensitivity of Fluorescent Porphyrin Dimers to Viscosity and Temperature.

Authors:  Aurimas Vyšniauskas; Dong Ding; Maryam Qurashi; Igor Boczarow; Milan Balaz; Harry L Anderson; Marina K Kuimova
Journal:  Chemistry       Date:  2017-06-13       Impact factor: 5.236

6.  Direct imaging of changes in aerosol particle viscosity upon hydration and chemical aging.

Authors:  N A Hosny; C Fitzgerald; A Vyšniauskas; A Athanasiadis; T Berkemeier; N Uygur; U Pöschl; M Shiraiwa; M Kalberer; F D Pope; M K Kuimova
Journal:  Chem Sci       Date:  2015-11-12       Impact factor: 9.825
  6 in total

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