| Literature DB >> 22821592 |
Andrew M Smith1, Tanja Durbic, Saranya Kittanakom, Guri Giaever, Corey Nislow.
Abstract
With the advent of next-generation sequencing (NGS) technology, methods previously developed for microarrays have been adapted for use by NGS. Here we describe in detail a protocol for Barcode analysis by sequencing (Bar-seq) to assess pooled competitive growth of individually barcoded yeast deletion mutants. This protocol has been optimized on two sequencing platforms: Illumina's Genome Analyzer IIx/HiSeq2000 and Life Technologies SOLiD3/5500. In addition, we provide guidelines for assessment of human knockdown cells using short-hairpin RNAs (shRNA) and an Illumina sequencing readout.Entities:
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Year: 2012 PMID： 22821592 DOI： 10.1007/978-1-61779-965-5_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745