Vasif Hasirci1, Brian J Pepe-Mooney. 1. Middle East Technical University, Biotechnology Research Unit, Ankara 06531, Turkey. vhasirci@metu.edu.tr
Abstract
AIM: This article reports on studies conducted in the same laboratory on interactions between patterned substrates with different pattern dimensions and chemistries, and various types of cells. MATERIALS & METHODS: In order to compare the influence of various parameters, bone marrow stromal cells, retinal pigment epithelial cells, human corneal stromal cells (keratocytes), Saos-2 (human osteosarcoma cells), human microvascular endothelial cells and vascular smooth muscle cells were tested on surfaces with different physical patterns and chemical properties. RESULTS: It was observed that cell type and surface topography are more influential than surface chemistry in determining the alignment tendency of a cell on a substrate surface. Low walls (several microns high) could not confine cells into the microgrooves of the films but alignment was still possible if the cells had a natural alignment property. CONCLUSION: This information is very useful in designing tissue engineering scaffolds and in the long-term success of implants.
AIM: This article reports on studies conducted in the same laboratory on interactions between patterned substrates with different pattern dimensions and chemistries, and various types of cells. MATERIALS & METHODS: In order to compare the influence of various parameters, bone marrow stromal cells, retinal pigment epithelial cells, human corneal stromal cells (keratocytes), Saos-2 (humanosteosarcoma cells), human microvascular endothelial cells and vascular smooth muscle cells were tested on surfaces with different physical patterns and chemical properties. RESULTS: It was observed that cell type and surface topography are more influential than surface chemistry in determining the alignment tendency of a cell on a substrate surface. Low walls (several microns high) could not confine cells into the microgrooves of the films but alignment was still possible if the cells had a natural alignment property. CONCLUSION: This information is very useful in designing tissue engineering scaffolds and in the long-term success of implants.