Literature DB >> 228058

Separation and characterization of herpes simplex virus type 1 immediate-early mRNA's.

R J Watson, C M Preston, J B Clements.   

Abstract

Polyadenylated immediate-early transcripts of herpes simplex virus type 1, made in BHK cells infected and maintained in the presence of cycloheximide, have been separated on denaturing agarose gels containing methyl mercuric hydroxide. Three virus-specific mRNA bands of estimated sizes 4.7, 3.0, and 2.0 kilobases (kb) were detected, and these mRNA's were mapped on the virus genome and also used to direct protein synthesis in vitro. The 4.7- and 3.0-kb mRNA's hybridized predominantly to certain DNA fragments which are located in the short and long repetitive regions of the genome, respectively, whereas the 2.0-kb mRNA's mapped to three discrete regions of the virus DNA. In vitro translation of these separated mRNA size classes indicated that the 3.0-kb mRNA specified the synthesis of virus polypeptide Vmw 110, whereas the 2.0-kb mRNA's specified Vmw 68, 63, and 12. The synthesis of small amounts of Vmw 175 was specified by the 4.7-kb mRNA. In contrast with the mRNA's which specify these other immediate-early polypeptides, that specifying Vmw 12 is much larger than required for its coding sequences.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 228058      PMCID： PMC353420     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  25 in total

1.  Recombinants between herpes simplex virus types 1 and 2: analyses of genome structures and expression of immediate early polypeptides.

Authors:  V G Preston; A J Davison; H S Marsden; M C Timbury; J H Subak-Sharpe; N M Wilkie
Journal:  J Virol       Date:  1978-11       Impact factor: 5.103

2.  Characterization of transcription-deficient temperature-sensitive mutants of herpes simplex virus type 1.

Authors:  R J Watson; J B Clements
Journal:  Virology       Date:  1978-12       Impact factor: 3.616

3.  Regulation of herpesvirus macromolecular synthesis. V. Properties of alpha polypeptides made in HSV-1 and HSV-2 infected cells.

Authors:  L Pereira; M H Wolff; M Fenwick; B Roizman
Journal:  Virology       Date:  1977-04       Impact factor: 3.616

4.  The cell-free synthesis of herpesvirus-induced polypeptides.

Authors:  C M Preston
Journal:  Virology       Date:  1977-05-01       Impact factor: 3.616

5.  Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 X HSV-2 recombinants.

Authors:  L S Morse; L Pereira; B Roizman; P A Schaffer
Journal:  J Virol       Date:  1978-05       Impact factor: 5.103

6.  Organization and expression of early genes of simian virus 40.

Authors:  L V Crawford; C N Cole; A E Smith; E Paucha; P Tegtmeyer; K Rundell; P Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1978-01       Impact factor: 11.205

7.  Large and small tumor antigens from simian virus 40 have identical amino termini mapping at 0.65 map units.

Authors:  E Paucha; A Mellor; R Harvey; A E Smith; R M Hewick; M D Waterfield
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205

8.  Cell-free synthesis of simian virus 40 T-antigens.

Authors:  E Paucha; R Harvey; A E Smith
Journal:  J Virol       Date:  1978-10       Impact factor: 5.103

9.  Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors:  G K McMaster; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

10.  Evidence that herpes simplex virus DNA is transcribed by cellular RNA polymerase B.

Authors:  F Costanzo; G Campadelli-Fiume; L Foa-Tomasi; E Cassai
Journal:  J Virol       Date:  1977-03       Impact factor: 5.103
View more
  90 in total

1.  Perturbation of cell cycle progression and cellular gene expression as a function of herpes simplex virus ICP0.

Authors:  W E Hobbs; N A DeLuca
Journal:  J Virol       Date:  1999-10       Impact factor: 5.103

2.  Efficient activation of viral genomes by levels of herpes simplex virus ICP0 insufficient to affect cellular gene expression or cell survival.

Authors:  W E Hobbs; D E Brough; I Kovesdi; N A DeLuca
Journal:  J Virol       Date:  2001-04       Impact factor: 5.103

3.  Mapping the termini and intron of the spliced immediate-early transcript of equine herpesvirus 1.

Authors:  R N Harty; C F Colle; F J Grundy; D J O'Callaghan
Journal:  J Virol       Date:  1989-12       Impact factor: 5.103

4.  Detection of herpes simplex virus type 1 transcripts during latent infection in mice.

Authors:  J G Spivack; N W Fraser
Journal:  J Virol       Date:  1987-12       Impact factor: 5.103

5.  Properties of cells carrying the herpes simplex virus type 2 thymidine kinase gene: mechanisms of reversion to a thymidine kinase-negative phenotype.

Authors:  K F Bastow; G Darby; P Wildy; A C Minson
Journal:  J Virol       Date:  1980-12       Impact factor: 5.103

6.  In vitro transcription and translation of proteins encoded by the BamHI-B genomic fragment of herpes simplex virus-1.

Authors:  M Moyal; Y Asher; G Darai; A Rösen-Wolff; A Vafai; Y Becker
Journal:  Virus Genes       Date:  1991-04       Impact factor: 2.332

7.  The UL5 gene of herpes simplex virus type 1: isolation of a lacZ insertion mutant and association of the UL5 gene product with other members of the helicase-primase complex.

Authors:  L A Zhu; S K Weller
Journal:  J Virol       Date:  1992-01       Impact factor: 5.103

8.  Codons 262 to 490 from the herpes simplex virus ICP4 gene are sufficient to encode a sequence-specific DNA binding protein.

Authors:  C L Wu; K W Wilcox
Journal:  Nucleic Acids Res       Date:  1990-02-11       Impact factor: 16.971

9.  The influence of the herpes simplex virus-1 DNA template environment on the regulation of gene expression.

Authors:  K Leary; H H Yim; L B Zhou; R E Sekulovich; R M Sandri-Goldin
Journal:  Virus Genes       Date:  1989-09       Impact factor: 2.332

10.  Requirements for activation of the herpes simplex virus glycoprotein C promoter in vitro by the viral regulatory protein ICP4.

Authors:  B Gu; N DeLuca
Journal:  J Virol       Date:  1994-12       Impact factor: 5.103
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.