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Literature DB >> 228054

Intracellular forms of simian virus 40 nucleoprotein complexes. II. Biochemical and electron microscopic analysis of simian virus 40 virion assembly.

Abstract

The simian virus 40 virion assembly process was studied with pulse-labeling kinetics of virion proteins, CsCl gradient analysis, electron microscopy, and low-salt gel electrophoresis. The results obtained are consistent with the model of gradual addition and organization of capsid proteins around simian virus 40 chromatin. Empty virions, as observed in the CsCl gradient by previous workers, were found to be the dissociation product of immature virus. Histone H1 was found in simian virus 40 chromatin and virion assembly intermediates but not in the mature virion banding at 1.34 g/ml in the CsCl gradient.

Entities: Chemical Species

Mesh：

Substances：
DNA, Viral
Viral Proteins

Year: 1979 PMID： 228054 PMCID： PMC353436

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

20 in total

1. Properties of the histones associated with simian virus 40 replicative form nucleoprotein complex.

Authors: J P MacGregor; Y H Chen; D A Goldstein; M R Hall
Journal: Biochem Biophys Res Commun Date: 1978-08-14 Impact factor: 3.575

2. Histones: metabolism in simian virus 40-infected cells and incorporation into virions.

Authors: K B Tan
Journal: Proc Natl Acad Sci U S A Date: 1977-07 Impact factor: 11.205

3. A technique of low-pH gel electrophoresis of chromosomal proteins which does not require preliminary removal of DNA.

Authors: V V Shmatchenko; A J Varshavsky
Journal: Anal Biochem Date: 1978-03 Impact factor: 3.365

4. Does simian virus 40 DNA integrate into cellular DNA during productive infection?

Authors: P W Rigby; P Berg
Journal: J Virol Date: 1978-11 Impact factor: 5.103

5. Chromosome of the mature virion of simian virus 40 contains H1 histone.

Authors: S A Nedospasov; V V Bakayev; G P Georgiev
Journal: Nucleic Acids Res Date: 1978-08 Impact factor: 16.971

6. Intracellular forms of simian virus 40 nucleoprotein complexes. I. Methods of isolation and characterization in CV-1 cells.

Authors: R Fernandez-Munoz; M Coca-Prados; M T Hsu
Journal: J Virol Date: 1979-02 Impact factor: 5.103

7. Synthesis and assembly of simian virus 40. II. Synthesis of the major capsid protein and its incorporation into viral particles.

Authors: H L Ozer; P Tegtmeyer
Journal: J Virol Date: 1972-01 Impact factor: 5.103

8. Synthesis and assembly of simian virus 40. I. Differential synthesis of intact virions and empty shells.

Authors: H L Ozer
Journal: J Virol Date: 1972-01 Impact factor: 5.103

9. Temperature-sensitive mutant of adenovirus type 2 blocked in virion assembly: accumulation of light intermediate particles.

Authors: J C D'Halluin; M Milleville; P A Boulanger; G R Martin
Journal: J Virol Date: 1978-05 Impact factor: 5.103

10. Saccharomyces cerevisiae plasmid, Scp or 2 mum: intracellular distribution, stability and nucleosomal-like packaging.

Authors: V L Seligy; D Y Thomas; B L Miki
Journal: Nucleic Acids Res Date: 1980-08-11 Impact factor: 16.971

Intracellular forms of simian virus 40 nucleoprotein complexes. II. Biochemical and electron microscopic analysis of simian virus 40 virion assembly.

1. Properties of the histones associated with simian virus 40 replicative form nucleoprotein complex.

2. Histones: metabolism in simian virus 40-infected cells and incorporation into virions.

3. A technique of low-pH gel electrophoresis of chromosomal proteins which does not require preliminary removal of DNA.

4. Does simian virus 40 DNA integrate into cellular DNA during productive infection?

5. Chromosome of the mature virion of simian virus 40 contains H1 histone.

6. Intracellular forms of simian virus 40 nucleoprotein complexes. I. Methods of isolation and characterization in CV-1 cells.

7. Synthesis and assembly of simian virus 40. II. Synthesis of the major capsid protein and its incorporation into viral particles.

8. Synthesis and assembly of simian virus 40. I. Differential synthesis of intact virions and empty shells.

9. Temperature-sensitive mutant of adenovirus type 2 blocked in virion assembly: accumulation of light intermediate particles.

10. Infectious linear DNA sequences replicating in simian virus 40-infected cells.

1. A structural rationale for SV40 Vp1 temperature-sensitive mutants and their complementation.

2. Simian Virus 40 Assembly, Studied by Temperature-Induced Conformational Changes in Capsid Protein VP1.

3. Characterization of the DNA-binding properties of the polyomavirus capsid protein VP1.

4. Epigenetic Analysis of SV40 Minichromosomes.

5. Role of the agnoprotein in regulation of simian virus 40 replication and maturation pathways.

6. Simian virus 40 large T antigen host range domain functions in virion assembly.

7. Intracellular forms of simian virus 40 nucleoprotein complexes. IV. Micrococcal nuclease digestion.

8. Simian virus 40 encapsidation: characterization of early intermediates.

9. Intracellular forms of simian virus 40 nucleoprotein complexes. III. Study of histone modifications.

10. Saccharomyces cerevisiae plasmid, Scp or 2 mum: intracellular distribution, stability and nucleosomal-like packaging.