Literature DB >> 228044

Uncapping of viral messenger RNA by phosphodiesterase of fibroblast plasma membranes.

S Harshman, J G Conlin, D Stoller, D L Harshman.   

Abstract

Isolated plasma membranes from mouse fibroblast lines 3T3 and its tranformant SV-3T3 contain a phosphodiesterase (oligonucleotidase, E.C. 3.1.4.19; nucleotide pyrophosphatase, E.C. 3.6.1.9) that splits capped and methylated messenger RNA obtained from both reovirus and vesicular stomatitis virus. The isolated membranes are free of demonstrable ribonuclease activity and split the mRNA to produce 7-methyl guanosine diphosphate as a product. With ATP as substrate for the phosphodiesterase enzyme, the product is AMP. Synthetic caps, AMP, ADP and ATP, but not cyclic AMP, can compete with the substrate p-nitrophenyl thymidilic acid. A possible regulatory role on messenger translation is proposed.

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Year:  1979        PMID： 228044     DOI： 10.1007/bf01868947

Source DB:  PubMed          Journal:  J Membr Biol        ISSN： 0022-2631            Impact factor:   1.843


  15 in total

1.  Detection in HeLa cell extracts of a 7-methyl guanosine specific enzyme activity that cleaves m7GpppNm.

Authors:  D L Nuss; Y Furuichi; G Koch; A J Shatkin
Journal:  Cell       Date:  1975-09       Impact factor: 41.582

2.  A simplified procedure for isolating plasma membranes from cultured mouse fibroblast cells: 3T3 and SV-3T3.

Authors:  S Harshman; J G Conlin
Journal:  Anal Biochem       Date:  1978-10-01       Impact factor: 3.365

3.  5'-Terminal 7-methylguanosine in eukaryotic mRNA is required for translation.

Authors:  S Muthukrishnan; G W Both; Y Furuichi; A J Shatkin
Journal:  Nature       Date:  1975-05-01       Impact factor: 49.962

4.  Translation and identification of the mRNA species synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus.

Authors:  G W Both; S A Moyer; A K Banerjee
Journal:  Proc Natl Acad Sci U S A       Date:  1975-01       Impact factor: 11.205

5.  Importance of 5'-terminal blocking structure to stabilize mRNA in eukaryotic protein synthesis.

Authors:  K Shimotohno; Y Kodama; J Hashimoto; K I Miura
Journal:  Proc Natl Acad Sci U S A       Date:  1977-07       Impact factor: 11.205

6.  Cycloleucine blocks 5'-terminal and internal methylations of avian sarcoma virus genome RNA.

Authors:  K Dimock; C M Stolzfus
Journal:  Biochemistry       Date:  1978-08-22       Impact factor: 3.162

Review 7.  Introductory remarks: membrane-polysome interactions.

Authors:  H C Pitot; T K Shires
Journal:  Fed Proc       Date:  1973-01

8.  Poly adenylic acid synthesis in vitro in isolated HeLa cell nuclei and whole cell homogenates,.

Authors:  W R Jelinek
Journal:  Cell       Date:  1974-07       Impact factor: 41.582

9.  Increase in plasma membrane phosphodiesterase activity in contact-inhibited 3T3 cells and in phenotypically reverted SV-3T3 cells.

Authors:  S Harshman; D Stoller; D L Harshman
Journal:  J Membr Biol       Date:  1979-10-15       Impact factor: 1.843

10.  Ribosome binding to reovirus mRNA in protein synthesis requires 5' terminal 7-methylguanosine.

Authors:  G W Both; Y Furuichi; S Muthukrishnan; A J Shatkin
Journal:  Cell       Date:  1975-10       Impact factor: 41.582
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  2 in total

1.  Use of adenosine 5'-0-(2-thiotriphosphate) for revealing of newly initiated transcripts in isolated rat liver nuclei.

Authors:  M Hanausek-Walaszek; Z Walaszek; M Chorazy
Journal:  Mol Biol Rep       Date:  1983-05       Impact factor: 2.316

2.  Increase in plasma membrane phosphodiesterase activity in contact-inhibited 3T3 cells and in phenotypically reverted SV-3T3 cells.

Authors:  S Harshman; D Stoller; D L Harshman
Journal:  J Membr Biol       Date:  1979-10-15       Impact factor: 1.843
  2 in total

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