Literature DB >> 22796839

Challenges of interferon-γ release assay conversions in serial testing of health-care workers in a TB control program.

Kimberlee S Fong1, J Walton Tomford2, Lucileia Teixeira2, Thomas G Fraser2, David van Duin2, Belinda Yen-Lieberman3, Steve M Gordon2, Cyndee Miranda2.   

Abstract

BACKGROUND: Clinical data with use of serial interferon-γ release assay (IGRA) testing in US health-care workers (HCWs) are limited.
METHODS: A single-center, retrospective chart review was done from 2007 to 2010 of HCWs who underwent preemployment QuantiFERON-TB Gold In-Tube testing. Demographic data, bacille Calmette-Guérin history, prior tuberculin skin test result if done, and baseline and serial IGRA values were obtained. The number of IGRA converters and reverters and their subsequent management by infectious disease physicians were reviewed. Quantitative IGRA-negative values were not available.
RESULTS: A total of 7,374 IGRAs were performed on newly hired HCWs. Of these tests, 486 (6.6%) were positive at baseline, 305 (4.1%) were indeterminate, and 6,583 (89.3%) were negative. From 2007 to 2010, 52 of 1,857 HCWs (2.8%) with serial IGRA tests were identified as converters, with a serial IGRA median value of 0.63 IU/mL. Seventy-one percent of HCWs with IGRA conversion had values ≤ 1 IU/mL. None of the converters had active TB or were part of an outbreak investigation.
CONCLUSIONS: Clinical significance of most QuantiFERON-TB Gold In-Tube conversions in serial testing remains a challenging task for clinicians. The use of a single cutoff point criterion for IGRA may lead to overdiagnosis of new TB infections. Clinical assessment and evaluation may help to prevent unnecessary therapy in these cases. The criteria for defining conversions and reversions by establishing new cutoffs needs to be evaluated further, especially in HCWs.

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Year:  2012        PMID: 22796839     DOI: 10.1378/chest.11-0992

Source DB:  PubMed          Journal:  Chest        ISSN: 0012-3692            Impact factor:   9.410


  27 in total

1.  Interferon-gamma release assays for screening of health care workers in low tuberculosis incidence settings: dynamic patterns and interpretational challenges.

Authors:  Madkuhar Pai; Kevin Elwood
Journal:  Can Respir J       Date:  2012 Mar-Apr       Impact factor: 2.409

2.  Challenges with QuantiFERON-TB Gold assay for large-scale, routine screening of U.S. healthcare workers.

Authors:  Madeline L Slater; Gary Welland; Madhukar Pai; Julie Parsonnet; Niaz Banaei
Journal:  Am J Respir Crit Care Med       Date:  2013-10-15       Impact factor: 21.405

Review 3.  Reproducibility of interferon gamma (IFN-γ) release Assays. A systematic review.

Authors:  Saloua Tagmouti; Madeline Slater; Andrea Benedetti; Sandra V Kik; Niaz Banaei; Adithya Cattamanchi; John Metcalfe; David Dowdy; Richard van Zyl Smit; Nandini Dendukuri; Madhukar Pai; Claudia Denkinger
Journal:  Ann Am Thorac Soc       Date:  2014-10

Review 4.  Gamma interferon release assays for detection of Mycobacterium tuberculosis infection.

Authors:  Madhukar Pai; Claudia M Denkinger; Sandra V Kik; Molebogeng X Rangaka; Alice Zwerling; Olivia Oxlade; John Z Metcalfe; Adithya Cattamanchi; David W Dowdy; Keertan Dheda; Niaz Banaei
Journal:  Clin Microbiol Rev       Date:  2014-01       Impact factor: 26.132

5.  Diagnosis and treatment of latent tuberculosis infection: an update.

Authors:  Anna K Person; April C Pettit; Timothy R Sterling
Journal:  Curr Respir Care Rep       Date:  2013-12

6.  Incidence of occupational latent tuberculosis infection in South African healthcare workers.

Authors:  Shahieda Adams; Rodney Ehrlich; Roslynn Baatjies; Richard N van Zyl-Smit; Qonita Said-Hartley; Rodney Dawson; Keertan Dheda
Journal:  Eur Respir J       Date:  2015-02-19       Impact factor: 16.671

7.  Two-Year Follow-up Study of Mycobacterium tuberculosis Antigen-Driven IFN-γ Responses and Macrophage sCD14 Levels After Tuberculosis Contact.

Authors:  Magdalena Druszczynska; Marcin Wlodarczyk; Grzegorz Kielnierowski; Malwina Kawka; Wieslawa Rudnicka
Journal:  Indian J Microbiol       Date:  2016-02-23       Impact factor: 2.461

8.  Test variability of the QuantiFERON-TB gold in-tube assay in clinical practice.

Authors:  John Z Metcalfe; Adithya Cattamanchi; Charles E McCulloch; Justin D Lew; Ngan P Ha; Edward A Graviss
Journal:  Am J Respir Crit Care Med       Date:  2012-10-26       Impact factor: 21.405

Review 9.  Practice Guidelines for Clinical Microbiology Laboratories: Mycobacteria.

Authors:  Betty A Forbes; Geraldine S Hall; Melissa B Miller; Susan M Novak; Marie-Claire Rowlinson; Max Salfinger; Akos Somoskövi; David M Warshauer; Michael L Wilson
Journal:  Clin Microbiol Rev       Date:  2018-01-31       Impact factor: 26.132

10.  Screening for latent tuberculosis in Norwegian health care workers: high frequency of discordant tuberculin skin test positive and interferon-gamma release assay negative results.

Authors:  Gerd Gran; Jörg Aßmus; Anne Ma Dyrhol-Riise
Journal:  BMC Public Health       Date:  2013-04-17       Impact factor: 3.295

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