Literature DB >> 22791367

Orosphere assay: a method for propagation of head and neck cancer stem cells.

Sudha Krishnamurthy1, Jacques E Nör.   

Abstract

BACKGROUND: Recent evidence suggests that head and neck squamous cell carcinomas (HNSCCs) harbor a small subpopulation of highly tumorigenic cells, designated cancer stem cells. A limiting factor in cancer stem cell research is the intrinsic difficulty of expanding cells in an undifferentiated state in vitro.
METHODS: Here, we describe the development of the orosphere assay, a method for the study of putative head and neck cancer stem cells. An orosphere is defined as a nonadherent colony of cells sorted from primary HNSCCs or from HNSCC cell lines and cultured in 3-dimensional soft agar or ultralow attachment plates. Aldehyde dehydrogenase activity and CD44 expression were used here as stem cell markers.
RESULTS: This assay allowed for the propagation of head and neck cancer cells that retained stemness and self-renewal.
CONCLUSION: The orosphere assay is well suited for studies designed to understand the pathobiology of head and neck cancer stem cells.
Copyright © 2012 Wiley Periodicals, Inc.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 22791367      PMCID： PMC3887391          DOI： 10.1002/hed.23076

Source DB:  PubMed          Journal:  Head Neck        ISSN： 1043-3074            Impact factor:   3.147


  30 in total

Review 1.  Stem cells: units of development, units of regeneration, and units in evolution.

Authors:  I L Weissman
Journal:  Cell       Date:  2000-01-07       Impact factor: 41.582

2.  In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells.

Authors:  Gabriela Dontu; Wissam M Abdallah; Jessica M Foley; Kyle W Jackson; Michael F Clarke; Mari J Kawamura; Max S Wicha
Journal:  Genes Dev       Date:  2003-05-15       Impact factor: 11.361

3.  Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system.

Authors:  B A Reynolds; S Weiss
Journal:  Science       Date:  1992-03-27       Impact factor: 47.728

4.  Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell.

Authors:  B A Reynolds; S Weiss
Journal:  Dev Biol       Date:  1996-04-10       Impact factor: 3.582

5.  Comparison of purity and enrichment of CD34+ cells from bone marrow, umbilical cord and peripheral blood (primed for apheresis) using five separation systems.

Authors:  E A de Wynter; L H Coutinho; X Pei; J C Marsh; J Hows; T Luft; N G Testa
Journal:  Stem Cells       Date:  1995-09       Impact factor: 6.277

Review 6.  Survival of mammary stem cells in suspension culture: implications for stem cell biology and neoplasia.

Authors:  Gabriela Dontu; Max S Wicha
Journal:  J Mammary Gland Biol Neoplasia       Date:  2005-01       Impact factor: 2.673

7.  Three-dimensional spheroid culture of human gingiva-derived mesenchymal stem cells enhances mitigation of chemotherapy-induced oral mucositis.

Authors:  Qunzhou Zhang; Andrew L Nguyen; Shihong Shi; Colin Hill; Petra Wilder-Smith; Tatiana B Krasieva; Anh D Le
Journal:  Stem Cells Dev       Date:  2011-07-28       Impact factor: 3.272

Review 8.  Growth and differentiation of the normal mammary gland and its tumours.

Authors:  P S Rudland; R Barraclough; D G Fernig; J A Smith
Journal:  Biochem Soc Symp       Date:  1998

9.  Identification of a lineage of multipotent hematopoietic progenitors.

Authors:  S J Morrison; A M Wandycz; H D Hemmati; D E Wright; I L Weissman
Journal:  Development       Date:  1997-05       Impact factor: 6.868

10.  Cancerous stem cells can arise from pediatric brain tumors.

Authors:  Houman D Hemmati; Ichiro Nakano; Jorge A Lazareff; Michael Masterman-Smith; Daniel H Geschwind; Marianne Bronner-Fraser; Harley I Kornblum
Journal:  Proc Natl Acad Sci U S A       Date:  2003-11-26       Impact factor: 11.205
View more
  33 in total

1.  Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype.

Authors:  Zhaocheng Zhang; Zhihong Dong; Isabel S Lauxen; Manoel Sant'Ana Filho; Jacques E Nör
Journal:  Cancer Res       Date:  2014-03-31       Impact factor: 12.701

2.  Isolation and Characterization of Cancer Stem Cells from Primary Head and Neck Squamous Cell Carcinoma Tumors.

Authors:  Hong S Kim; Alexander T Pearson; Jacques E Nör
Journal:  Methods Mol Biol       Date:  2016

3.  Effects of extracellular acidity on resistance to chemotherapy treatment: a systematic review.

Authors:  Viviane Palmeira da Silva; Carulina Bueno Mesquita; Júlia Silveira Nunes; Bianca de Bem Prunes; Pantelis Varvaki Rados; Fernanda Visioli
Journal:  Med Oncol       Date:  2018-10-30       Impact factor: 3.064

Review 4.  Modeling head and neck cancer stem cell-mediated tumorigenesis.

Authors:  Alexander T Pearson; Trachette L Jackson; Jacques E Nör
Journal:  Cell Mol Life Sci       Date:  2016-05-05       Impact factor: 9.261

Review 5.  Strategies for isolating and enriching cancer stem cells: well begun is half done.

Authors:  Jiang-Jie Duan; Wen Qiu; Sen-Lin Xu; Bin Wang; Xian-Zong Ye; Yi-Fang Ping; Xia Zhang; Xiu-Wu Bian; Shi-Cang Yu
Journal:  Stem Cells Dev       Date:  2013-05-09       Impact factor: 3.272

6.  Endothelial-Initiated Crosstalk Regulates Dental Pulp Stem Cell Self-Renewal.

Authors:  M Oh; Z Zhang; A Mantesso; A E Oklejas; J E Nör
Journal:  J Dent Res       Date:  2020-05-29       Impact factor: 6.116

7.  Head and neck cancer organoids established by modification of the CTOS method can be used to predict in vivo drug sensitivity.

Authors:  Noriaki Tanaka; Abdullah A Osman; Yoko Takahashi; Antje Lindemann; Ameeta A Patel; Mei Zhao; Hideaki Takahashi; Jeffrey N Myers
Journal:  Oral Oncol       Date:  2018-10-23       Impact factor: 5.337

8.  SCF/C-Kit Signaling Induces Self-Renewal of Dental Pulp Stem Cells.

Authors:  Carolina Cucco; Zhaocheng Zhang; Tatiana M Botero; Daniel J Chiego; Rogerio M Castilho; Jacques E Nör
Journal:  J Endod       Date:  2020-09       Impact factor: 4.171

Review 9.  Oral epithelial stem cells - implications in normal development and cancer metastasis.

Authors:  Silvana Papagerakis; Giuseppe Pannone; Li Zheng; Imad About; Nawar Taqi; Nghia P T Nguyen; Margarite Matossian; Blake McAlpin; Angela Santoro; Jonathan McHugh; Mark E Prince; Petros Papagerakis
Journal:  Exp Cell Res       Date:  2014-05-05       Impact factor: 3.905

10.  Role of miRNA dynamics and cytokine profile in governing CD44v6/Nanog/PTEN axis in oral cancer: modulating the master regulators.

Authors:  Shanaya Patel; Rakesh Rawal
Journal:  Tumour Biol       Date:  2016-09-09
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.