Historical control data from 13-week repeated toxicity studies in Crj:CD (SD) rats.

Reference ranges of standard experimental parameters are useful for comparisons in toxicology. The aim of this study was to collect data from 13-week repeated toxicity studies in Crl:CD (SD) rats, a strain widely used for toxicity and efficacy research, for establishing domestic reference values. Data on body weight, food consumption; urinalysis, hematological, and blood biochemical parameters; and organ weights were collected from 11 toxicity studies in 220 Crl:CD (SD) rats (110 males and 110 females). The studies had been performed at a single testing facility over the last 5 years and involved animals sourced from a single breeder. The findings were collated as means, standard deviations, percentages, and ranges. Urine volume, uterus weight, eosinophil, and basophil counts, and triglyceride, total bilirubin, and gamma-glutamyl transpeptidase levels showed standard deviations of 30% or more. These historical control data would help to interpret the effects of test substances in routine toxicity and efficacy studies.

The toxicity and efficacy of food additives, pharmaceuticals, and chemicals have been interpreted on the basis of background data collected from laboratory animals. Although these data are invaluable and essential, they are affected by age, breeding environment, feeding, measurement equipment, and testing method [1,2]. The results of toxicity and efficacy studies have also been interpreted by establishing control groups. Nevertheless, such results are difficult to interpret when the control data are not consistent or when the experimental values are extremes of the control data [3]. In addition, sporadic changes unrelated to the test substance or false-positive results are common. In such cases, the toxicological significance is generally determined by checking for the presence of a dose response, changes in related parameters, or changes in physiological ranges [4]. Background data are used to determine various physiological ranges. Understanding such data is important because it is the first step for appropriate interpretation of treatment-related changes [1]. However, the current major animal breeders do not provide background data on standard experimental parameters. Further, the results of many studies have been interpreted on the basis of foreign background data. Reference values in the published domestic literature are scarce, and the available background data present information only of gender differences at the same age [5,6]. In addition, these data do not fully reflect the factors affecting data quality, because of sample sizes of less than 100 animals and collection periods of less than 1 year [7].

To the best of our knowledge, published background data from studies in rats is limited in Korea. In this study, we aimed to collect data from 13-week repeated toxicity studies in Crl:CD (SD) rats, a strain widely used for toxicity and efficacy research, for establishing domestic reference values.

Materials and Methods

Data collection

The data were gathered from 13-week repeated toxicity studies in 220 Crl:CD (SD) rats from same breeder, ages (ORIENTBIO INC., Korea). The studies had been performed in the same environment at a single testing facility (Biotoxtech Co. Ltd.) over the last 5 years and involved animals sourced from a single breeder.

Quarantine and acclimation

All animals had been quarantined and acclimated for 6-7 days, and their general health had been observed daily. Their body weights had been recorded on the day of receipt and group assignment by using an electronic balance (CP3202S, Sartorius AG, Goettingen, Germany). On the final day of quarantine and acclimation, their body weights had been recorded again, and no abnormality in clinical signs and body weight had been noted.

Animal husbandry

The animals had been housed individually in hanging stainless-steel wire mesh cages in an animal room under the following conditions: temperature, 19-25℃; relative humidity, 30.0-70.0%; air ventilation, 10-15 times/h; and illumination, 12 h (0700-1900 h). They had been fed commercial diet (Teklad Certified Irradiated Global 18% Protein Rodent Diet 2918C).

Method of administration

The animals had received injection water (JW Pharmaceutical, Korea) once daily by gastric intubation using 3-mL disposable syringes fitted with intubation tubes during the indicated dosing period.

Urinalysis

The rats had been fasted during urine collection, but drinking water was available ad libitum. Urine volume had been measured with a calibrated cylinder. Urine samples had been examined for protein, glucose, ketone bodies, urobilinogen, bilirubin, occult blood, and nitrite by using reagent strips and a urine analyzer (Miditron Junior II, Roche Diagnostics, Basel, Switzerland).

Hematology

Blood samples had been collected from the abdominal aorta into collection tubes containing EDTA-2K. However, for determining coagulation parameters, blood samples treated with 3.2% sodium citrate water had been used. The animals had been fasted for approximately 18 h before sampling. The following hematological tests had been performed by using an automated hematology analyzer (ADVIA 120, Siemens Healthcare Diagnostics, Tarrytown, NY, USA): erythrocyte count, hemoglobin level, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and platelet, leukocyte, neutrophil, lymphocyte, monocyte, eosinophil, basophil, and reticulocyte counts. Prothrombin time, activated partial thromboplastin time, and fibrinogen level had been measured with a coagulation analyzer (ACL 7000, Instrumentation Laboratory, Bedford, USA).

Blood biochemistry

After centrifugation, the following blood biochemical tests had been performed by using an automated blood chemistry analyzer (7080, Hitachi, Tokyo, Japan): alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma-glutamyl transpeptidase, blood urea nitrogen, creatinine, total bilirubin, total protein, albumin, total cholesterol, triglyceride, phosphorus, glucose, and calcium. Chloride, sodium, and potassium levels had been measured with an electrolyte analyzer (AVL 9181, Roche Diagnostics, Basel, Switzerland).

Organ weights

All animals had been sacrificed and examined macroscopically, and their organ weighed recorded of receipt and group assignment by using an electronic balance (CP3202S, Sartorius AG, Goettingen, Germany).

Results

Body weight

The male and female rats showed a body weight gain of 298% and 203%, respectively, from week 1 to week 13 (Figure 1). The standard deviation among individuals of the same age was within 10% of the mean body weight. Male rats tended to gain more than 10% body weight from the first to the third experimental week. However, the body weight reached a plateau from week 4 to week 13. Female rats showed a similar trend.

Figure 1

Changes in body weight of male and female rats

Food consumption

Male rats consumed the maximum amount of food in the seventh experimental week; thereafter, food consumption reached a plateau until the end of the study. Similarly, female rats showed the maximum food consumption in the fifth week, which then remained constant until the end of the study (Figure 2). Male and Female rats consumed 26.6-33.8 and 20.7-23.9 g/day respectively. The greatest difference between the minimum and the maximum consumption by male rats occurred in the fourth experimental week (27.1-49.6 g/day). In female rats, the widest range was 12.0-26.5 g/day in the first experimental week.

Figure 2

Changes in food consumption of male and female rats

Urinalysis data

Male rats demonstrated the presence of ketone bodies, protein, and occult blood in urine, and female rats had proteinuria. The proportions of positive results in male and female rats were as follows: protein, 42 and 20%, respectively; ketone bodies, 12 and 2%, respectively; and occult blood, 4 and 2%, respectively. In addition, the standard deviation of urine volume was over 30% of the mean value in both genders (Table 1).

Table 1

Urinary parameter of male and female rats

Parentheses represent incidence

Hematological data

The standard deviations of erythrocyte count, hemoglobin level, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration were less than 10% of the mean values in both male and female rats (Table 2). For platelet and reticulocyte counts, the standard deviations were more than 10% and less than 20% of the mean values in male and female rats, respectively. Further, the standard deviation of lymphocyte count was less than 10% of the mean value in female rats and more than 10% but less than 20% of the mean value in male rats. The standard deviations of the other differential counts were more than 30%. The standard deviation of prothrombin time was less than 10% of the mean value in both gender. Finally, the standard deviations of activated partial thromboplastin time and fibrinogen level in male rats were more than 10% and less than 20% of the mean values.

Table 2

Hematological parameter of male and female rats

RBC, erythrocyte count; HGB, hemoglobin; HCT, hematocrit; Reti, reticulocyte; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; PLT, platelet; WBC, leukocyte; NEW, neutrophil; LYM, lymphocyte; MONO, monocyte; EOS, eosinophil; BASO, basophil; PT, prothrombin time; APTT, activated partial thromboplastin time; Fib, fibrinogen

Blood biochemical data

The alkaline phosphatase and triglyceride levels showed gender differences: alkaline phosphatase, 290.3±52.2 U/L in male rats and 150.3±41.9 U/L in female rats; triglyceride, 67±31 mg/dL in male rats and 32±14 mg/dL in female rats (Table 3). The standard deviations of chloride, sodium, and potassium, calcium, albumin, and total protein levels in male and female rats were less than 10% of the mean values. The standard deviations of A/G ratio and phosphorus level in male rats and glucose level in female rats were less than 10% of the mean values. Further, the standard deviations of glucose, blood urea nitrogen, and creatinine levels and A/G ratio were more than 10% and less than 20% of the mean values in male and female rats. The standard deviations of total cholesterol and alanine aminotransferase levels in female rats were more than 10% and less than 20% of the mean values. Moreover, the standard deviations of total cholesterol level in male and female rats, and aspartate aminotransferase, alkaline phosphatase, alanine aminotransferase levels in male rats were more than 20% and less than 30% of the mean values. Finally, the standard deviations of gamma-glutamyl transpeptidase, total bilirubin, and triglyceride levels in male and female rats, and aspartate aminotransferase, alkaline phosphatase, alanine aminotransferase levels in female rats were more than 30% of the mean values.

Table 3

Blood chemistry parameter of male and female rats

ALT, alanine aminotransferase; AST, aspartate aminotransferase; ALP, alkaline phosphatase; GGT, gamma-glutamyl transpeptidase; GLU, glucose; BUN, blood urea nitrogen; Crea, creatinine; T-Bili, total bilirubin; T-Chol, total chloesterol;TG, triglyceride; TP, total protein; Alb, albumin; A/G, albumin/globulin; P, phosphorus, Ca, calcium; Na, sodium; K, potassium; Cl, Chlorde

The standard deviations of brain, heart, lung, kidney, and testis weights were less than 10% of the mean values (Table 4). Further, the standard deviations of pituitary, liver, spleen, and adrenal weights were more than 10% and less than 20% of the mean values. The standard deviation of prostate weight was more than 20% and less than 30% of the mean value, and that of uterus weight was more than 30% of the mean value.

Table 4

Organ weight of male and female rats

NA: Not applicable

Discussion

In this study, we collated means, standard deviations, percentages, and ranges of body weight, food consumption, urinalysis, hematological and blood biochemical parameter, and organ weights of Crl:CD (SD) rats from 11 studies of 13-week repeated toxicity to establish domestic reference values.

Body weight is an important parameter indicating the animal's condition. It is the basis to determine the dose of anesthetics or test substances in toxicity studies [8]. However, this parameter is frequently affected by feeding, measurement time, and measurement sensitivity. In our study, the standard deviations of body weight at each age were less than 10%; thus, individual differences were small.

Food consumption is a sensitive parameter because reduced food intake by normal-appearing animals suggests loss of appetite induced by the test substance. However, food consumption is frequently affected by individual differences and measurement time. In the current study, the standard deviations of food consumption at each age were more than 10% and less than 20% of the mean values, indicating individual variations. The average food intake decreased from week 7 in male rats and week 8 in female rats. Increased food intake during growth and decreased food intake during maturation are associated with changes in metabolism. The tendency observed in our study is similar to other reports [9].

Urinalysis data are indicators of kidney disease. Urinalysis is a useful tool for accessing a longitudinal effect of a test substance by repetitive sampling [10,11]. Urinalysis data are indicators of kidney disease, but they are frequently affected by stress due to environmental changes such as oliguria or contamination with feces or pellets [12,13]. In our study, male rats tended to show higher positive values than female rats [14,15]. In addition, the standard deviation of over 30% of the average urine volume in both genders suggests great individual differences. Gender differences in urine protein are reportedly caused by differences in sex hormones [16,17].

Blood acts as a carrier of food and oxygen to cells, waste away from cells, and various disease-fighting cells such as leukocytes. It is also a means of "puncture proofing" the body: it clots and seals small holes quickly. Further, it is important for maintaining a constant body temperature. However, hematological parameters are frequently affected by collection site, storage method, fasting period, anesthetics, anticoagulants, and measurement method [18]. In the current study, the standard deviations of these parameters at each age were more than 30%, indicating great individual differences. The analyzed parameters showed a similar tendency to that previously reported [19].

Blood chemistry provides specific information about bodily functions including metabolism, drug absorption, blood circulation, and fluid balance. However, the analyzed parameters are frequently affected by collection site, storage method, fasting period, and anesthetics [20,21]. In our study, the standard deviations of gamma-glutamyl transpeptidase, total bilirubin, and triglyceride levels in male and female rats, and alanine amino-transferase, aspartate aminotransferase, and alkaline phosphatase levels in female rats were more than 30% of the mean values, again suggesting great individual differences. All blood biochemical parameters tended to show similar changes to those reported previously [3,9]. The variations in liver-associated enzymatic activity could be related to enzymatic variations due to age, gender, and other factors [22,23].

Organ weight is used to determine the effect of a test substance in toxicity studies. However, this parameter is frequently affected by trimming method, measurement time, and measurement sensitivity. In the current study, the standard deviations of organ weights at each age were more than 30%, implying great individual differences, and similar to previously reported findings [3].

Differences in analytical methods as well as environmental and technique-related variables influence the obtained parametric values. As the data in this study were obtained from a single testing facility by using animals sourced from a single breeder, many of these variables would have been eliminated or minimized. Therefore, these historical control data would help to interpret the effects of test substances in routine toxicity and efficacy studies.