BACKGROUND: The mRNA expression of genes coding enzymes involved in O-GlcNAcylation were analyzed in urine obtained from 176 bladder cancer (BC) patients and 143 healthy persons. METHODS: MGEA5 and OGT expression was measured by a real-time PCR assay. RESULTS: OGT expression was not detected in urine of healthy persons but it was found in 51.7% of BC samples. Positive expression of MGEA5 was found in urine of both healthy persons (47.1%) and BC patients (52.3%). Poorly differentiated BC (grade III) showed significantly lower MGEA5 expression than grade I tumors. Contrary, OGT transcript level was significantly higher in grade II and III in comparison to grade I BC. Moreover, there was significant difference in OGT expression between early bladder cancers and invasive or advanced bladder cancers. CONCLUSIONS: These results suggest that analysis of urinary content of MGEA5 and OGT may be useful for bladder cancer diagnostics.
BACKGROUND: The mRNA expression of genes coding enzymes involved in O-GlcNAcylation were analyzed in urine obtained from 176 bladder cancer (BC) patients and 143 healthy persons. METHODS:MGEA5 and OGT expression was measured by a real-time PCR assay. RESULTS:OGT expression was not detected in urine of healthy persons but it was found in 51.7% of BC samples. Positive expression of MGEA5 was found in urine of both healthy persons (47.1%) and BC patients (52.3%). Poorly differentiated BC (grade III) showed significantly lower MGEA5 expression than grade I tumors. Contrary, OGT transcript level was significantly higher in grade II and III in comparison to grade I BC. Moreover, there was significant difference in OGT expression between early bladder cancers and invasive or advanced bladder cancers. CONCLUSIONS: These results suggest that analysis of urinary content of MGEA5 and OGT may be useful for bladder cancer diagnostics.
Authors: Valerie L Sodi; Sakina Khaku; Raisa Krutilina; Luciana P Schwab; David J Vocadlo; Tiffany N Seagroves; Mauricio J Reginato Journal: Mol Cancer Res Date: 2015-01-30 Impact factor: 5.852
Authors: Andréia Vasconcelos-Dos-Santos; Isadora A Oliveira; Miguel Clodomiro Lucena; Natalia Rodrigues Mantuano; Stephen A Whelan; Wagner Barbosa Dias; Adriane Regina Todeschini Journal: Front Oncol Date: 2015-06-25 Impact factor: 6.244