Literature DB >> 22780320

Effective flow cytometric phenotyping of cells using minimal amounts of antibody.

Deepak Sharma1, Mark R Eichelberg, Jill D Haag, Amanda L Meilahn, Matthew J Muelbl, Kathy Schell, Bart M G Smits, Michael N Gould.   

Abstract

Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproducibly accurate results for the detection of up to seven parameters for activation induced-proliferation, cell cycle analysis, and phenotyping of cell-surface and intracellular antigens.

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Year:  2012        PMID： 22780320      PMCID： PMC3523330          DOI： 10.2144/0000113854

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  14 in total

1.  Differences in antigen recognition and cytolytic activity of CD8(+) and CD8(-) T cells that express the same antigen-specific receptor.

Authors:  B K Cho; K C Lian; P Lee; A Brunmark; C McKinley; J Chen; D M Kranz; H N Eisen
Journal:  Proc Natl Acad Sci U S A       Date:  2001-02-13       Impact factor: 11.205

2.  Purification and unique properties of mammary epithelial stem cells.

Authors:  John Stingl; Peter Eirew; Ian Ricketson; Mark Shackleton; François Vaillant; David Choi; Haiyan I Li; Connie J Eaves
Journal:  Nature       Date:  2006-01-04       Impact factor: 49.962

Review 3.  Interleukin-7 receptor expression: intelligent design.

Authors:  Renata Mazzucchelli; Scott K Durum
Journal:  Nat Rev Immunol       Date:  2007-02       Impact factor: 53.106

Review 4.  Keeping abreast of the mammary epithelial hierarchy and breast tumorigenesis.

Authors:  Jane E Visvader
Journal:  Genes Dev       Date:  2009-11-15       Impact factor: 11.361

5.  Differential expression of CD3 and CD7 in T-cell malignancies: a quantitative study by flow cytometry.

Authors:  L Ginaldi; E Matutes; N Farahat; M De Martinis; R Morilla; D Catovsky
Journal:  Br J Haematol       Date:  1996-06       Impact factor: 6.998

Review 6.  Anatomy of the antibody molecule.

Authors:  E A Padlan
Journal:  Mol Immunol       Date:  1994-02       Impact factor: 4.407

7.  Evidence for an association between CD8 molecules and the T cell receptor complex on cytotoxic T cells.

Authors:  S Takada; E G Engleman
Journal:  J Immunol       Date:  1987-11-15       Impact factor: 5.422

8.  Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part I: Panel design by an empiric approach.

Authors:  Bridget E McLaughlin; Nicole Baumgarth; Martin Bigos; Mario Roederer; Stephen C De Rosa; John D Altman; Douglas F Nixon; Janet Ottinger; Carol Oxford; Thomas G Evans; David M Asmuth
Journal:  Cytometry A       Date:  2008-05       Impact factor: 4.714

9.  Quantification of epithelial cell differentiation in mammary glands and carcinomas from DMBA- and MNU-exposed rats.

Authors:  Deepak Sharma; Bart M G Smits; Mark R Eichelberg; Amanda L Meilahn; Matthew J Muelbl; Jill D Haag; Michael N Gould
Journal:  PLoS One       Date:  2011-10-12       Impact factor: 3.240

10.  The non-protein coding breast cancer susceptibility locus Mcs5a acts in a non-mammary cell-autonomous fashion through the immune system and modulates T-cell homeostasis and functions.

Authors:  Bart M G Smits; Deepak Sharma; David J Samuelson; Stephan Woditschka; Bob Mau; Jill D Haag; Michael N Gould
Journal:  Breast Cancer Res       Date:  2011-08-16       Impact factor: 6.466
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  3 in total

1.  A magnetic micropore chip for rapid (<1 hour) unbiased circulating tumor cell isolation and in situ RNA analysis.

Authors:  Jina Ko; Neha Bhagwat; Stephanie S Yee; Taylor Black; Colleen Redlinger; Janae Romeo; Mark O'Hara; Arjun Raj; Erica L Carpenter; Ben Z Stanger; David Issadore
Journal:  Lab Chip       Date:  2017-09-12       Impact factor: 6.799

2.  Microfluidics for the detection of minimal residual disease in acute myeloid leukemia patients using circulating leukemic cells selected from blood.

Authors:  Joshua M Jackson; James B Taylor; Małgorzata A Witek; Sally A Hunsucker; Jennifer P Waugh; Yuri Fedoriw; Thomas C Shea; Steven A Soper; Paul M Armistead
Journal:  Analyst       Date:  2016-01-21       Impact factor: 4.616

3.  The gene desert mammary carcinoma susceptibility locus Mcs1a regulates Nr2f1 modifying mammary epithelial cell differentiation and proliferation.

Authors:  Bart M G Smits; Jill D Haag; Anna I Rissman; Deepak Sharma; Ann Tran; Alexi A Schoenborn; Rachael C Baird; Dan S Peiffer; David Q Leinweber; Matthew J Muelbl; Amanda L Meilahn; Mark R Eichelberg; Ning Leng; Christina Kendziorski; Manorama C John; Patricia A Powers; Caroline M Alexander; Michael N Gould
Journal:  PLoS Genet       Date:  2013-06-13       Impact factor: 5.917
  3 in total

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