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Literature DB >> 22772730

Multicolor two-photon tissue imaging by wavelength mixing.

Pierre Mahou¹, Maxwell Zimmerley, Karine Loulier, Katherine S Matho, Guillaume Labroille, Xavier Morin, Willy Supatto, Jean Livet, Delphine Débarre, Emmanuel Beaurepaire.

Abstract

We achieve simultaneous two-photon excitation of three chromophores with distinct absorption spectra using synchronized pulses from a femtosecond laser and an optical parametric oscillator. The two beams generate separate multiphoton processes, and their spatiotemporal overlap provides an additional two-photon excitation route, with submicrometer overlay of the color channels. We report volume and live multicolor imaging of 'Brainbow'-labeled tissues as well as simultaneous three-color fluorescence and third-harmonic imaging of fly embryos.

Mesh：

Year: 2012 PMID： 22772730 DOI： 10.1038/nmeth.2098

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

28 in total

1. Resolution of multiple green fluorescent protein color variants and dyes using two-photon microscopy and imaging spectroscopy.

Authors: R Lansford; G Bearman; S E Fraser
Journal: J Biomed Opt Date: 2001-07 Impact factor: 3.170

2. Depth-resolved structural imaging by third-harmonic generation microscopy.

Authors: Dan Oron; Dvir Yelin; Eran Tal; Sefi Raz; Rachel Fachima; Yaron Silberberg
Journal: J Struct Biol Date: 2004-07 Impact factor: 2.867

Review 3. Sparse and combinatorial neuron labelling.

Authors: Gregory S X E Jefferis; Jean Livet
Journal: Curr Opin Neurobiol Date: 2011-10-24 Impact factor: 6.627

Review 4. The big and the small: challenges of imaging the brain's circuits.

Authors: Jeff W Lichtman; Winfried Denk
Journal: Science Date: 2011-11-04 Impact factor: 47.728

Review 5. Deep tissue two-photon microscopy.

Authors: Fritjof Helmchen; Winfried Denk
Journal: Nat Methods Date: 2005-12 Impact factor: 28.547

6. Deep and fast live imaging with two-photon scanned light-sheet microscopy.

Authors: Thai V Truong; Willy Supatto; David S Koos; John M Choi; Scott E Fraser
Journal: Nat Methods Date: 2011-07-17 Impact factor: 28.547

7. Generating and imaging multicolor Brainbow mice.

Authors: Tamily A Weissman; Joshua R Sanes; Jeff W Lichtman; Jean Livet
Journal: Cold Spring Harb Protoc Date: 2011-07-01

8. Two-color two-photon excitation of fluorescence.

Authors: J R Lakowicz; I Gryczynski; H Malak; Z Gryczynski
Journal: Photochem Photobiol Date: 1996-10 Impact factor: 3.421

9. Quantitative imaging of collective cell migration during Drosophila gastrulation: multiphoton microscopy and computational analysis.

Authors: Willy Supatto; Amy McMahon; Scott E Fraser; Angelike Stathopoulos
Journal: Nat Protoc Date: 2009-09-10 Impact factor: 13.491

10. Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging.

Authors: David Entenberg; Jeffrey Wyckoff; Bojana Gligorijevic; Evanthia T Roussos; Vladislav V Verkhusha; Jeffrey W Pollard; John Condeelis
Journal: Nat Protoc Date: 2011-09-08 Impact factor: 13.491

64 in total

1. Two-photon imaging of multiple fluorescent proteins by phase-shaping and linear unmixing with a single broadband laser.

Authors: Meredith H Brenner; Dawen Cai; Joel A Swanson; Jennifer P Ogilvie
Journal: Opt Express Date: 2013-07-15 Impact factor: 3.894

10. Combination of an optical parametric oscillator and quantum-dots 655 to improve imaging depth of vasculature by intravital multicolor two-photon microscopy.

Authors: Clément Ricard; Lisa Lamasse; Alexandre Jaouen; Geneviève Rougon; Franck Debarbieux
Journal: Biomed Opt Express Date: 2016-05-24 Impact factor: 3.732

Multicolor two-photon tissue imaging by wavelength mixing.

1. Resolution of multiple green fluorescent protein color variants and dyes using two-photon microscopy and imaging spectroscopy.

2. Depth-resolved structural imaging by third-harmonic generation microscopy.

Review 3. Sparse and combinatorial neuron labelling.

Review 4. The big and the small: challenges of imaging the brain's circuits.

Review 5. Deep tissue two-photon microscopy.

6. Deep and fast live imaging with two-photon scanned light-sheet microscopy.

7. Generating and imaging multicolor Brainbow mice.

8. Two-color two-photon excitation of fluorescence.

9. Quantitative imaging of collective cell migration during Drosophila gastrulation: multiphoton microscopy and computational analysis.

10. Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging.

1. Two-photon imaging of multiple fluorescent proteins by phase-shaping and linear unmixing with a single broadband laser.

Review 2. Brainbow: new resources and emerging biological applications for multicolor genetic labeling and analysis.

3. Measurement of the relative non-degenerate two-photon absorption cross-section for fluorescence microscopy.

4. Synchronously pumped Raman laser for simultaneous degenerate and nondegenerate two-photon microscopy.

5. Non-degenerate 2-photon excitation in scattering medium for fluorescence microscopy.

6. Simple approach to three-color two-photon microscopy by a fiber-optic wavelength convertor.

7. Frontiers in Intravital Multiphoton Microscopy of Cancer.

8. Multicolor two-photon light-sheet microscopy.

Review 9. Advances in whole-embryo imaging: a quantitative transition is underway.

10. Combination of an optical parametric oscillator and quantum-dots 655 to improve imaging depth of vasculature by intravital multicolor two-photon microscopy.