BACKGROUND: The use of botulinum toxin type A (BoNT) continues to expand. Some physicians have noted a face-lifting effect after intradermal injection of BoNT, although the effects are controversial. OBJECTIVE: To investigate the in vitro effects of BoNT on human dermal fibroblasts. METHODS: The proliferation and toxic effects of BoNT on human dermal fibroblasts were measured. To understand the mechanism of BoNT on collagen production of fibroblasts, procollagen type I carboxy-terminal peptide (PIP) was measured using enzyme-linked immunosorbent assay, and collagen production was monitored using Western blotting. To examine the effect of BoNT on collagen degradation, we evaluated matrix metalloproteinase (MMP) production using gelatin zymography. RESULTS: BoNT did not stimulate the proliferation of or show toxic effects on human dermal fibroblasts. Levels of PIP increased significantly in fibroblasts grown in the presence of BoNT, and BoNT upregulated the expression of type I collagen and decreased the production of some MMPs in fibroblasts that prevent collagen degradation. CONCLUSIONS: This study shows interesting effects of BoNT on collagen production and degradation of human dermal fibroblasts in vitro. This research provides the experimental background for using intradermal BoNT injection for remodeling of dermal tissues in aged skin.
BACKGROUND: The use of botulinum toxin type A (BoNT) continues to expand. Some physicians have noted a face-lifting effect after intradermal injection of BoNT, although the effects are controversial. OBJECTIVE: To investigate the in vitro effects of BoNT on human dermal fibroblasts. METHODS: The proliferation and toxic effects of BoNT on human dermal fibroblasts were measured. To understand the mechanism of BoNT on collagen production of fibroblasts, procollagen type I carboxy-terminal peptide (PIP) was measured using enzyme-linked immunosorbent assay, and collagen production was monitored using Western blotting. To examine the effect of BoNT on collagen degradation, we evaluated matrix metalloproteinase (MMP) production using gelatin zymography. RESULTS: BoNT did not stimulate the proliferation of or show toxic effects on human dermal fibroblasts. Levels of PIP increased significantly in fibroblasts grown in the presence of BoNT, and BoNT upregulated the expression of type I collagen and decreased the production of some MMPs in fibroblasts that prevent collagen degradation. CONCLUSIONS: This study shows interesting effects of BoNT on collagen production and degradation of human dermal fibroblasts in vitro. This research provides the experimental background for using intradermal BoNT injection for remodeling of dermal tissues in aged skin.
Authors: Cindy Bandala; Juan Luis Terán-Melo; Maricruz Anaya-Ruiz; Cesar Miguel Mejía-Barradas; Rene Domínguez-Rubio; Paloma De la Garza-Montano; Alfonso Alfaro-Rodríguez; Eleazar Lara-Padilla Journal: Int J Clin Exp Pathol Date: 2015-08-01
Authors: Ying-Ying Miao; Juan Liu; Jie Zhu; Yan-Ling Tao; Jia-An Zhang; Dan Luo; Bing-Rong Zhou Journal: Biomed Res Int Date: 2017-02-07 Impact factor: 3.411
Authors: Jie Zhu; Xi Ji; Min Li; Xiao-e Chen; Juan Liu; Jia-an Zhang; Dan Luo; Bing-rong Zhou Journal: Biomed Res Int Date: 2016-02-22 Impact factor: 3.411