Literature DB >> 22735947

Yeast display of engineered antibody domains.

Qi Zhao1, Zhongyu Zhu, Dimiter S Dimitrov.   

Abstract

Yeast display is an efficient technology for selection of antibodies and other proteins with high affinity and thermal stability. Here, we describe a method for affinity maturation of engineered antibody domains (eAds) using yeast display. EAd yeast libraries of relatively large size (∼10⁹) were generated and subjected to alternating rounds of magnetic-activated cell sorting (MACS), fluorescent-activated cell sorting (FACS), and random mutagenesis. The highest affinity clones from the final round of maturation were identified and analyzed. We discuss extensively each key step, and provide detailed protocols and helpful notes.

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Year:  2012        PMID： 22735947      PMCID： PMC6957256          DOI： 10.1007/978-1-61779-921-1_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Directed evolution of soluble single-chain human class II MHC molecules.

Authors:  Olga Esteban; Huimin Zhao
Journal:  J Mol Biol       Date:  2004-06-25       Impact factor: 5.469

2.  Isolating and engineering human antibodies using yeast surface display.

Authors:  Ginger Chao; Wai L Lau; Benjamin J Hackel; Stephen L Sazinsky; Shaun M Lippow; K Dane Wittrup
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

3.  Efficient recovery of high-affinity antibodies from a single-chain Fab yeast display library.

Authors:  Laura M Walker; Diana R Bowley; Dennis R Burton
Journal:  J Mol Biol       Date:  2009-04-16       Impact factor: 5.469

4.  Picomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shuffling.

Authors:  Benjamin J Hackel; Atul Kapila; K Dane Wittrup
Journal:  J Mol Biol       Date:  2008-06-24       Impact factor: 5.469

Review 5.  Engineered CH2 domains (nanoantibodies).

Authors:  Dimiter S Dimitrov
Journal:  MAbs       Date:  2009 Jan-Feb       Impact factor: 5.857

6.  An improved yeast transformation method for the generation of very large human antibody libraries.

Authors:  Lorenzo Benatuil; Jennifer M Perez; Jonathan Belk; Chung-Ming Hsieh
Journal:  Protein Eng Des Sel       Date:  2010-02-03       Impact factor: 1.650

7.  A large library based on a novel (CH2) scaffold: identification of HIV-1 inhibitors.

Authors:  Xiaodong Xiao; Yang Feng; Bang K Vu; Rieko Ishima; Dimiter S Dimitrov
Journal:  Biochem Biophys Res Commun       Date:  2009-07-15       Impact factor: 3.575

8.  Development of a novel strategy for engineering high-affinity proteins by yeast display.

Authors:  S A Richman; S J Healan; K S Weber; D L Donermeyer; M L Dossett; P D Greenberg; P M Allen; D M Kranz
Journal:  Protein Eng Des Sel       Date:  2006-03-20       Impact factor: 1.650

9.  Engineered human antibody constant domains with increased stability.

Authors:  Rui Gong; Bang K Vu; Yang Feng; DaRue A Prieto; Marzena A Dyba; Joseph D Walsh; Ponraj Prabakaran; Timothy D Veenstra; Sergey G Tarasov; Rieko Ishima; Dimiter S Dimitrov
Journal:  J Biol Chem       Date:  2009-03-23       Impact factor: 5.157

Review 10.  Adnectins: engineered target-binding protein therapeutics.

Authors:  D Lipovsek
Journal:  Protein Eng Des Sel       Date:  2010-11-10       Impact factor: 1.650
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  7 in total

1.  Alteration of Electrostatic Surface Potential Enhances Affinity and Tumor Killing Properties of Anti-ganglioside GD2 Monoclonal Antibody hu3F8.

Authors:  Qi Zhao; Mahiuddin Ahmed; Hong-fen Guo; Irene Y Cheung; Nai-Kong V Cheung
Journal:  J Biol Chem       Date:  2015-04-07       Impact factor: 5.157

Review 2.  Applications of Yeast Surface Display for Protein Engineering.

Authors:  Gerald M Cherf; Jennifer R Cochran
Journal:  Methods Mol Biol       Date:  2015

3.  N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies.

Authors:  Dezhi Li; Jie Liu; Li Zhang; Tianshu Xu; Junheng Chen; Liping Wang; Qi Zhao
Journal:  Virol Sin       Date:  2015-12-21       Impact factor: 4.327

4.  Engineered antibody CH2 domains binding to nucleolin: Isolation, characterization and improvement of aggregation.

Authors:  Dezhi Li; Rui Gong; Jun Zheng; Xihai Chen; Dimiter S Dimitrov; Qi Zhao
Journal:  Biochem Biophys Res Commun       Date:  2017-02-13       Impact factor: 3.575

Review 5.  Discovery of novel candidate therapeutics and diagnostics based on engineered human antibody domains.

Authors:  Weizao Chen; Rui Gong; Tianlei Ying; Ponraj Prabakaran; Zhongyu Zhu; Yang Feng; Dimiter S Dimitrov
Journal:  Curr Drug Discov Technol       Date:  2014-03

6.  Affinity maturation of T-cell receptor-like antibodies for Wilms tumor 1 peptide greatly enhances therapeutic potential.

Authors:  Q Zhao; M Ahmed; D V Tassev; A Hasan; T-Y Kuo; H-F Guo; R J O'Reilly; N-K V Cheung
Journal:  Leukemia       Date:  2015-05-19       Impact factor: 11.528

7.  A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells.

Authors:  Zhizhen Chen; Jie Liu; Dafeng Chu; Yaming Shan; Guixing Ma; Hongmin Zhang; Xiaohua Douglas Zhang; Pu Wang; Qiang Chen; Chuxia Deng; Weizao Chen; Dimiter S Dimitrov; Qi Zhao
Journal:  Int J Biol Sci       Date:  2018-05-21       Impact factor: 6.580
  7 in total

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