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Literature DB >> 2273262

The use of tetramethylbenzidine for solid phase immunoassays.

Abstract

A rapid, sensitive, solid-phase immunoassay, using horseradish peroxidase and 3,3',5,5'-tetramethylbenzidine, was found to be more sensitive and the colour developed was more stable compared with HRPO using 4-chloro-1-naphthol or diaminobenzidine. Assay sensitivity was equal to or greater than that obtained with the alkaline phosphatase reaction. The essential step was to incubate filters in 1% dextran sulphate for 10 min in a pH 5 (10 mM citrate-EDTA) buffer before reacting with TMB (0.05 mg/ml in the same buffer).

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Year: 1990 PMID： 2273262 DOI： 10.1016/0022-1759(90)90282-z

Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303

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Cited

8 in total

1. Generation and characterization of variants of NWS/G70C influenza virus after in vitro passage in 4-amino-Neu5Ac2en and 4-guanidino-Neu5Ac2en.

Authors: J L McKimm-Breschkin; T J Blick; A Sahasrabudhe; T Tiong; D Marshall; G J Hart; R C Bethell; C R Penn
Journal: Antimicrob Agents Chemother Date: 1996-01 Impact factor: 5.191

2. Mechanism of fusion triggering by human parainfluenza virus type III: communication between viral glycoproteins during entry.

Authors: Matteo Porotto; Samantha G Palmer; Laura M Palermo; Anne Moscona
Journal: J Biol Chem Date: 2011-11-22 Impact factor: 5.157

3. Vaccination and protection of pigs against pleuropneumonia with a vaccine strain of Actinobacillus pleuropneumoniae produced by site-specific mutagenesis of the ApxII operon.

Authors: C T Prideaux; C Lenghaus; J Krywult; A L Hodgson
Journal: Infect Immun Date: 1999-04 Impact factor: 3.441

4. Protection of sheep against caseous lymphadenitis by use of a single oral dose of live recombinant Corynebacterium pseudotuberculosis.

Authors: A L Hodgson; M Tachedjian; L A Corner; A J Radford
Journal: Infect Immun Date: 1994-12 Impact factor: 3.441

5. Human parainfluenza virus infection of the airway epithelium: viral hemagglutinin-neuraminidase regulates fusion protein activation and modulates infectivity.

Authors: Laura M Palermo; Matteo Porotto; Christine C Yokoyama; Samantha G Palmer; Bruce A Mungall; Olga Greengard; Stefan Niewiesk; Anne Moscona
Journal: J Virol Date: 2009-04-22 Impact factor: 5.103

The use of tetramethylbenzidine for solid phase immunoassays.

1. Generation and characterization of variants of NWS/G70C influenza virus after in vitro passage in 4-amino-Neu5Ac2en and 4-guanidino-Neu5Ac2en.

2. Mechanism of fusion triggering by human parainfluenza virus type III: communication between viral glycoproteins during entry.

3. Vaccination and protection of pigs against pleuropneumonia with a vaccine strain of Actinobacillus pleuropneumoniae produced by site-specific mutagenesis of the ApxII operon.

4. Protection of sheep against caseous lymphadenitis by use of a single oral dose of live recombinant Corynebacterium pseudotuberculosis.

5. Human parainfluenza virus infection of the airway epithelium: viral hemagglutinin-neuraminidase regulates fusion protein activation and modulates infectivity.

6. A generic system for the expression and purification of soluble and stable influenza neuraminidase.

7. Glucose oxidase-mediated polymerization as a platform for dual-mode signal amplification and biodetection.

8. Taking down the FLAG! How insect cell expression challenges an established tag-system.