BACKGROUND: Remodeling of structural bone allografts relies on adequate revascularization, which can theoretically be induced by surgical revascularization. We developed a new orthotopic animal model to determine the technical feasibility of axial arteriovenous bundle implantation and resultant angiogenesis. QUESTIONS/PURPOSES: We asked whether arteriovenous bundles implanted in segmental allografts would increase cortical blood flow and angiogenesis compared to nonrevascularized frozen bone allografts and contralateral femoral controls. METHODS: We performed segmental femoral allotransplantation orthotopically from 10 Brown Norway rats to 20 Lewis rats. Ten rats each received either bone allograft reconstruction alone (Group I) or allograft combined with an intramedullary saphenous arteriovenous flap (Group II). At 16 weeks, we measured cortical blood flow with the hydrogen washout method. We then quantified angiogenesis using capillary density and micro-CT vessel volume measurements. RESULTS: All arteriovenous bundles were patent. Group II had higher mean blood flow (0.12 mL/minute/100 g versus 0.05 mL/minute/100 g), mean capillary density (23.6% versus 2.8%), and micro-CT vessel volume (0.37 mm(3) versus 0.07 mm(3)) than Group I. Revascularized allografts had higher capillary density than untreated contralateral femora, while vessel volume did not differ and blood flow was lower. CONCLUSIONS: Axial surgical revascularization in orthotopic allotransplants can achieve strong angiogenesis and increases cortical bone blood flow.
BACKGROUND: Remodeling of structural bone allografts relies on adequate revascularization, which can theoretically be induced by surgical revascularization. We developed a new orthotopic animal model to determine the technical feasibility of axial arteriovenous bundle implantation and resultant angiogenesis. QUESTIONS/PURPOSES: We asked whether arteriovenous bundles implanted in segmental allografts would increase cortical blood flow and angiogenesis compared to nonrevascularized frozen bone allografts and contralateral femoral controls. METHODS: We performed segmental femoral allotransplantation orthotopically from 10 Brown Norway rats to 20 Lewis rats. Ten rats each received either bone allograft reconstruction alone (Group I) or allograft combined with an intramedullary saphenous arteriovenous flap (Group II). At 16 weeks, we measured cortical blood flow with the hydrogen washout method. We then quantified angiogenesis using capillary density and micro-CT vessel volume measurements. RESULTS: All arteriovenous bundles were patent. Group II had higher mean blood flow (0.12 mL/minute/100 g versus 0.05 mL/minute/100 g), mean capillary density (23.6% versus 2.8%), and micro-CT vessel volume (0.37 mm(3) versus 0.07 mm(3)) than Group I. Revascularized allografts had higher capillary density than untreated contralateral femora, while vessel volume did not differ and blood flow was lower. CONCLUSIONS: Axial surgical revascularization in orthotopic allotransplants can achieve strong angiogenesis and increases cortical bone blood flow.
Authors: Yang-Guk Chung; Allen T Bishop; Goetz A Giessler; Osami Suzuki; Jeffrey L Platt; Michael Pelzer; Patricia F Friedrich; Thomas Kremer Journal: Xenotransplantation Date: 2010 Jan-Feb Impact factor: 3.907
Authors: D Donati; S Giacomini; E Gozzi; Y Salphale; M Mercuri; Henry J Mankin; Dempsy S Springfield; Mark C Gebhardt Journal: Clin Orthop Relat Res Date: 2002-07 Impact factor: 4.176
Authors: Noortje J Visser; Elisa S Rezaie; Patricia F Friedrich; Dimitra Kotsougiani; Alexander Y Shin; Allen T Bishop Journal: J Orthop Res Date: 2019-05-17 Impact factor: 3.494
Authors: Yunzhi Peter Yang; Benjamin C Gadomski; Arnaud Bruyas; Jeremiah Easley; Kevin M Labus; Brad Nelson; Ross H Palmer; Holly Stewart; Kirk McGilvray; Christian M Puttlitz; Dan Regan; Alexander Stahl; Elaine Lui; Jiannan Li; Seyedsina Moeinzadeh; Sungwoo Kim; William Maloney; Michael J Gardner Journal: Tissue Eng Part A Date: 2021-06-11 Impact factor: 3.845
Authors: Toshiyuki Kawai; Chi-Chun Pan; Yaichiro Okuzu; Takayoshi Shimizu; Alexander M Stahl; Shuich Matsuda; William J Maloney; Yunzhi P Yang Journal: Tissue Eng Part A Date: 2021-06-18 Impact factor: 3.845