OBJECTIVE: To compare the effects of N-acetylcysteine (NAC) and ethyl pyruvate (EP) on experimental testicular ischemia-reperfusion (I/R) injury. DESIGN: Randomized, controlled, experimental study. SETTING: University hospital. ANIMAL(S): Twenty-four mature male Wistar rats. INTERVENTION(S): Rats were divided into four groups: control group, torsion-detorsion (T/D) group, EP group, and NAC group. In the pretreatment of the NAC and EP groups, 20 mg/kg NAC and 50 mg/kg EP were given intraperitoneally (IP) 30 minutes before detorsion. MAIN OUTCOME MEASURE(S): Serum ischemia-modified albumin (IMA), tissue and serum malondialdehyde, and myeloperoxidase activity levels and histopathological damage scores were then compared. RESULT(S): Ethyl pyruvate and N-acetylcysteine exhibited a protective effect against I/R injury. Of the biochemical parameters evaluated as a result of testicular I/R, only IMA levels were significantly elevated. There was a strong and significant correlation between serum IMA levels and histopathological injury scores, and the increase in serum IMA level exhibited a strong parallel with the increase in histopathological injury. In the EP group, although the histopathological injury score was similar to that of the control group, serum IMA levels were significantly elevated. CONCLUSION(S): Both NAC and EP, the effects of which on I/R injury are evaluated in the present study, reduce such injury in testicular torsion-detorsion. Comparing their effects on IMA levels, NAC may be regarded as a relatively more effective treatment than EP.
OBJECTIVE: To compare the effects of N-acetylcysteine (NAC) and ethyl pyruvate (EP) on experimental testicular ischemia-reperfusion (I/R) injury. DESIGN: Randomized, controlled, experimental study. SETTING: University hospital. ANIMAL(S): Twenty-four mature male Wistar rats. INTERVENTION(S): Rats were divided into four groups: control group, torsion-detorsion (T/D) group, EP group, and NAC group. In the pretreatment of the NAC and EP groups, 20 mg/kg NAC and 50 mg/kg EP were given intraperitoneally (IP) 30 minutes before detorsion. MAIN OUTCOME MEASURE(S): Serum ischemia-modified albumin (IMA), tissue and serum malondialdehyde, and myeloperoxidase activity levels and histopathological damage scores were then compared. RESULT(S): Ethyl pyruvate and N-acetylcysteine exhibited a protective effect against I/R injury. Of the biochemical parameters evaluated as a result of testicular I/R, only IMA levels were significantly elevated. There was a strong and significant correlation between serum IMA levels and histopathological injury scores, and the increase in serum IMA level exhibited a strong parallel with the increase in histopathological injury. In the EP group, although the histopathological injury score was similar to that of the control group, serum IMA levels were significantly elevated. CONCLUSION(S): Both NAC and EP, the effects of which on I/R injury are evaluated in the present study, reduce such injury in testicular torsion-detorsion. Comparing their effects on IMA levels, NAC may be regarded as a relatively more effective treatment than EP.
Authors: Omar Al Hussein Alawamlh; Ryan Flannigan; Russell Hayden; Marc Goldstein; Philip S Li; Richard K Lee Journal: Adv Exp Med Biol Date: 2021 Impact factor: 2.622
Authors: Jonathan Poels; Gaël Abou-Ghannam; Sophie Herman; Anne Van Langendonckt; François-Xavier Wese; Christine Wyns Journal: Front Surg Date: 2014-12-02
Authors: Federico Del Vento; Maxime Vermeulen; Francesca de Michele; Maria Grazia Giudice; Jonathan Poels; Anne des Rieux; Christine Wyns Journal: Int J Mol Sci Date: 2018-01-18 Impact factor: 5.923