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Literature DB >> 2270083

Mutant of the glutamine transfer RNA gene as UGA suppressor in Escherichia coli.

H Inokuchi¹, K Kondo, M Yoshimura, H Ozeki.

Abstract

A UGA suppressor derived from a glutamine tRNA gene of Escherichia coli K12 was isolated and characterized. Phages carrying the suppressor su+2UGA could be obtained only from a hybrid transducing phage, h80cI857psu+2oc, but not from the original transducing phage lambda cI857psu+2oc. By DNA sequence analysis, it was found that the su+2 UGA suppressor obtained has two mutations; one is in the anticodon (TTA----TCA), as expected, and the other (C----T) is at the 7th position from the 3' end of tRNA(2Gln). The significance of these mutations and the lethal effect on phage lambda of the increased amounts of UGA suppressor tRNAs are discussed.

Entities: Chemical Species

Mesh：

Substances：
RNA, Transfer, Gln

Year: 1990 PMID： 2270083 DOI： 10.1007/bf00264450

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

15 in total

1. Identification of transfer RNA suppressors in Escherichia coli. I. Amber suppressor su+2, an anticodon mutant of tRNA2Gln.

Authors: H Inokuchi; F Yamao; H Sakano; H Ozeki
Journal: J Mol Biol Date: 1979-08-25 Impact factor: 5.469

2. Identification of transfer RNA suppressors in Escherichia coli. II. Duplicate genes for tRNA2Gln.

Authors: H Inokuchi; M Kodaira; F Yamao; H Ozeki
Journal: J Mol Biol Date: 1979-08-25 Impact factor: 5.469

Review 3. tRNA identity.

Authors: J Normanly; J Abelson
Journal: Annu Rev Biochem Date: 1989 Impact factor: 23.643

4. Mutational alterations of tryptophan-specific transfer RNA that generate translation suppressors of the UAA, UAG and UGA nonsense codons.

Authors: L Soll
Journal: J Mol Biol Date: 1974-06-25 Impact factor: 5.469

Mutant of the glutamine transfer RNA gene as UGA suppressor in Escherichia coli.

1. Identification of transfer RNA suppressors in Escherichia coli. I. Amber suppressor su+2, an anticodon mutant of tRNA2Gln.

2. Identification of transfer RNA suppressors in Escherichia coli. II. Duplicate genes for tRNA2Gln.

Review 3. tRNA identity.

4. Mutational alterations of tryptophan-specific transfer RNA that generate translation suppressors of the UAA, UAG and UGA nonsense codons.

5. Identification of an ochre-suppressing anticodon.

6. Duplicate genes for tyrosine transfer RNA in Escherichia coli.

7. Nucleotide sequence of bacteriophage lambda DNA.

8. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

9. Organization and structure of an E. coli tRNA operon containing seven tRNA genes.

10. DNA sequencing with chain-terminating inhibitors.

1. Switching tRNA(Gln) identity from glutamine to tryptophan.

2. Functional communication in the recognition of tRNA by Escherichia coli glutaminyl-tRNA synthetase.

3. New nucleotide sequence data on the EMBL File Server.