BACKGROUND: ITMN-191 (RG7227, Danoprevir), as a potential inhibitor of the NS3/4A protease of hepatitis C virus, has been in phase 2 clinical trial. Unfortunately, several ITMN-191 resistance mutants including R155K, A156V, and D168A/E have been identified. METHODS: Molecular dynamics simulation, binding free energy calculation and per-residue energy decomposition were employed to explore the binding and resistance mechanism of hepatitis C virus NS3/4A protease to ITMN-191. RESULTS: Based on molecular dynamics simulation and per-residue energy decomposition, the nonpolar energy term was found to be the driving force for ITMN-191 binding. For the studied R155K, A156V, D168A/E mutants, the origin of resistance is mainly from the conformational changes of the S4 and extended S2 binding pocket induced by the studied mutants and further leading to the reduced binding ability to the extended P2 and P4 moieties of ITMN-191. CONCLUSIONS: Further structural analysis indicates that the destruction of conservative salt bridges between residues 168 and 155 should be responsible for the large conformation changes of the binding pocket in R155K and D168A/E mutants. For A156V mutation, the occurrence of drug resistance is mainly from the changed binding pocket by a replacement of one bulky residue Val. GENERAL SIGNIFICANCE: The obtained drug resistance mechanism of this study will provide useful guidance for the development of new and effective HCV NS3/4A inhibitors with low resistance.
BACKGROUND:ITMN-191 (RG7227, Danoprevir), as a potential inhibitor of the NS3/4A protease of hepatitis C virus, has been in phase 2 clinical trial. Unfortunately, several ITMN-191 resistance mutants including R155K, A156V, and D168A/E have been identified. METHODS: Molecular dynamics simulation, binding free energy calculation and per-residue energy decomposition were employed to explore the binding and resistance mechanism of hepatitis C virusNS3/4A protease to ITMN-191. RESULTS: Based on molecular dynamics simulation and per-residue energy decomposition, the nonpolar energy term was found to be the driving force for ITMN-191 binding. For the studied R155K, A156V, D168A/E mutants, the origin of resistance is mainly from the conformational changes of the S4 and extended S2 binding pocket induced by the studied mutants and further leading to the reduced binding ability to the extended P2 and P4 moieties of ITMN-191. CONCLUSIONS: Further structural analysis indicates that the destruction of conservative salt bridges between residues 168 and 155 should be responsible for the large conformation changes of the binding pocket in R155K and D168A/E mutants. For A156V mutation, the occurrence of drug resistance is mainly from the changed binding pocket by a replacement of one bulky residue Val. GENERAL SIGNIFICANCE: The obtained drug resistance mechanism of this study will provide useful guidance for the development of new and effective HCV NS3/4A inhibitors with low resistance.
Authors: Djadé I Soumana; Nese Kurt Yilmaz; Kristina L Prachanronarong; Cihan Aydin; Akbar Ali; Celia A Schiffer Journal: ACS Chem Biol Date: 2016-01-06 Impact factor: 5.100
Authors: Hadas Dvory-Sobol; Kelly A Wong; Karin S Ku; Andrew Bae; Eric J Lawitz; Phillip S Pang; Jeanette Harris; Michael D Miller; Hongmei Mo Journal: Antimicrob Agents Chemother Date: 2012-08-06 Impact factor: 5.191
Authors: Mitchell Kramer; Daniel Halleran; Moazur Rahman; Mazhar Iqbal; Muhammad Ikram Anwar; Muhmad Ikram Anwar; Salwa Sabet; Edward Ackad; Mohammad S Yousef; Mohammad Yousef Journal: PLoS One Date: 2014-08-11 Impact factor: 3.240