| Literature DB >> 22688715 |
Laszlo Hackler1, Tomohiro Masuda, Verity F Oliver, Shannath L Merbs, Donald J Zack.
Abstract
Laser capture microdissection (LCM) is a useful method to isolate specific cells or cell layers of interest from heterogeneous tissues, such as the retina. The collected cells can be used for DNA, RNA, or protein analysis. We have applied LCM technology to isolate cells from the outer nuclear, inner nuclear, and ganglion cell layers of the retina for mRNA and microRNA (miRNA) expression and epigenetic (DNA methylation) analysis. Here, we describe the methods we have employed for sample preparation, LCM-based isolation of retinal layers, RNA/DNA extraction, RNA quality check, microRNA analysis by quantitative PCR, and DNA methylation analysis by bisulfite sequencing.Entities:
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Year: 2012 PMID： 22688715 PMCID： PMC4154361 DOI： 10.1007/978-1-61779-848-1_21
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745