| Literature DB >> 2267963 |
P H van Vianen1, S Thaithong, P P Reinders, A van Engen, M van der Keur, H J Tanke, H J van der Kaay, B Mons.
Abstract
A method is described for the fully automated reading of Plasmodium falciparum drug susceptibility tests. Cultured material was fixed and could be stored for greater than or equal to 6 months until analysis. The parasites were stained for DNA with the fluorescent dye Hoechst 33258 and analyzed by flow cytometry. The procedure was done in 96-well microtiter plates, after which the material was directed through the sensing region in the flow cytometer. The data resulting from the analysis were stored by microcomputer and processed by a program developed for this purpose. Using this method, a number of different parameters describing the growth in culture can be assessed.Entities:
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Year: 1990 PMID： 2267963 DOI： 10.4269/ajtmh.1990.43.602
Source DB: PubMed Journal: Am J Trop Med Hyg ISSN： 0002-9637 Impact factor: 2.345