Literature DB >> 22677266

Comparison of normalization methods for measuring immune markers in cervical secretion specimens.

Morgan A Marks1, Yolanda Eby, Roslyn Howard, Patti E Gravitt.   

Abstract

Ophthalmic sponges are used to collect undiluted cervical secretions for assessment of markers of genital tract immunity. Heterogeneity in absorbed and extracted sample volumes requires normalization in order to make valid inter-individual comparisons. We evaluated the performance of adjustment by weight and total protein on normalizing inter-individual variability of immune marker measurement due to differences in volume collection. Normalization to total protein resulted in a minimal loss of usable specimens and a significant reduction in the correlation of immune marker concentration to specimen weight compared to weight adjustment. Total protein normalization appeared to be more effective than weight adjustment in reducing the dependence of cervical immune marker concentrations on differences in specimen volume.
Copyright © 2012. Published by Elsevier B.V.

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Year:  2012        PMID： 22677266      PMCID： PMC3831520          DOI： 10.1016/j.jim.2012.05.012

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  12 in total

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Review 2.  Innate and adaptive immunity in female genital tract: cellular responses and interactions.

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4.  Differences in the concentration and correlation of cervical immune markers among HPV positive and negative perimenopausal women.

Authors:  Morgan A Marks; Raphael P Viscidi; Kathryn Chang; Michelle Silver; Anne Burke; Roslyn Howard; Patti E Gravitt
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5.  Comparative analysis of methods for collection and measurement of immunoglobulins in cervical and vaginal secretions of women.

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6.  Comparative analysis of methods for collection and measurement of cytokines and immunoglobulins in cervical and vaginal secretions of HIV and HPV infected women.

Authors:  I V Snowhite; W E Jones; J Dumestre; K Dunlap; P S Braly; M E Hagensee
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9.  Comparison of ophthalmic sponges for measurements of immune markers from cervical secretions.

Authors:  Philip E Castle; Ana-Cecilia Rodriguez; Frederick P Bowman; Rolando Herrero; Mark Schiffman; M Concepcion Bratti; Lidia Ana Morera; Danny Schust; Peggy Crowley-Nowick; Allan Hildesheim
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5.  Soil-Transmitted Helminth Infections Are Associated With an Increase in Human Papillomavirus Prevalence and a T-Helper Type 2 Cytokine Signature in Cervical Fluids.

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6.  Randomized Cross-Sectional Study to Compare HIV-1 Specific Antibody and Cytokine Concentrations in Female Genital Secretions Obtained by Menstrual Cup and Cervicovaginal Lavage.

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7.  Optimising the collection of female genital tract fluid for cytokine analysis in pregnant women.

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10.  Vaginal cytokine profile and microbiota before and after lubricant use compared with condomless vaginal sex: a preliminary observational study.

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  10 in total

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