| Literature DB >> 22664182 |
Gabriella Elia1, Costantina Desario, Giulia Pezzoni, Michele Camero, Emiliana Brocchi, Nicola Decaro, Vito Martella, Canio Buonavoglia.
Abstract
The gene encoding the VP2 protein of canine parvovirus type 2 was expressed in an insect-baculovirus system. The recombinant (r) VP2 was similar antigenically/functionally to the native capsid protein as demonstrated by hemagglutination, Western blotting and hemagglutination inhibition test, using Canine parvovirus type-2 (CPV-2) positive sera. An enzyme-linked immunosorbent assay (ELISA) using the rVP2 was used for testing CPV-2 positive and negative sera from dogs and for determining the threshold of maternally derived antibodies interfering with successful vaccination of pups against CPV-2.Entities:
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Year: 2012 PMID： 22664182 DOI： 10.1016/j.jviromet.2012.05.025
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014