Shewanella litorisediminis sp. nov., a gammaproteobacterium isolated from a tidal flat sediment.

A Gram-negative, facultatively anaerobic, non-motile and rod-shaped bacterial strain, designated SMK1-12(T), was isolated from a tidal flat sediment on the western coast of Korea. Phylogenetic analyses based on 16S rRNA and gyrB gene sequences showed that strain SMK1-12(T) belonged to the genus Shewanella, clustering with the type strain of Shewanella amazonensis. Strain SMK1-12(T) exhibited the highest 16S rRNA gene sequence similarity value (97.0 %) and the highest gyrB sequence similarity value (87.8 %) to S. amazonensis SB2B(T), respectively. Strain SMK1-12(T) contained simultaneously both menaquinones and ubiquinones; the predominant menaquinone was MK-7 and the predominant ubiquinones were Q-7 and Q-8. The major fatty acids (>10 % of the total fatty acids) detected in strain SMK1-12(T) were the MIDI system summed feature 3 (iso-C(15:0) 2-OH and/or C(16:1) ω7c), iso-C(15:0), C(17:1) ω8c and C(16:0). The DNA G+C content of strain SMK1-12(T) was 58.0 mol% and its mean DNA-DNA relatedness value with S. amazonensis ATCC 700329(T) was 15 ± 4.6 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SMK1-12(T) is distinguishable from recognized Shewanella species. On the basis of the data presented, strain SMK1-12(T) is considered to represent a novel Shewanella species, for which the name Shewanella litorisediminis sp. nov. is proposed. The type strain is SMK1-12(T) (=KCTC 23961(T) = CCUG 62411(T)).