Literature DB >> 22660718

Exploiting a natural auxotrophy for genetic selection.

Elizabeth Ramage1, Larry Gallagher, Colin Manoil.   

Abstract

We exploited the natural histidine auxotrophy of Francisella species to develop hisD (encodes histidinol dehydrogenase) as a positive selection marker. A shuttle plasmid (pBR103) carrying Escherichia coli hisD and designed for cloning of PCR fragments replicated in both attenuated and highly virulent Francisella strains. During this work, we formulated a simplified defined growth medium for Francisella novicida.

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Year:  2012        PMID： 22660718      PMCID： PMC3406110          DOI： 10.1128/AEM.00762-12

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


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