Literature DB >> 22652433

High-resolution melt PCR analysis for rapid identification of Chlamydia abortus live vaccine strain 1B among C. abortus strains and field isolates.

Fabien Vorimore1, Noémie Cavanna, Nadia Vicari, Simone Magnino, Hermann Willems, Annie Rodolakis, Victoria I Siarkou, Karine Laroucau.   

Abstract

We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus live vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.
Copyright © 2012 Elsevier B.V. All rights reserved.

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Year:  2012        PMID: 22652433     DOI: 10.1016/j.mimet.2012.05.014

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  3 in total

Review 1.  Recent advances and public health implications for environmental exposure to Chlamydia abortus: from enzootic to zoonotic disease.

Authors:  Lauretta Turin; Sara Surini; Nick Wheelhouse; Mara Silvia Rocchi
Journal:  Vet Res       Date:  2022-05-31       Impact factor: 3.829

2.  Diversification and Distribution of Ruminant Chlamydia abortus Clones Assessed by MLST and MLVA.

Authors:  Victoria I Siarkou; Fabien Vorimore; Nadia Vicari; Simone Magnino; Annie Rodolakis; Yvonne Pannekoek; Konrad Sachse; David Longbottom; Karine Laroucau
Journal:  PLoS One       Date:  2015-05-22       Impact factor: 3.240

3.  Genomic evidence that the live Chlamydia abortus vaccine strain 1B is not attenuated and has the potential to cause disease.

Authors:  David Longbottom; Michelle Sait; Morag Livingstone; Karine Laroucau; Konrad Sachse; Simon R Harris; Nicholas R Thomson; Helena M B Seth-Smith
Journal:  Vaccine       Date:  2018-06-14       Impact factor: 3.641

  3 in total

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