Literature DB >> 22642368

Synthesis of bispecific antibodies using genetically encoded unnatural amino acids.

Chan Hyuk Kim1, Jun Y Axup, Anna Dubrovska, Stephanie A Kazane, Benjamin A Hutchins, Erik D Wold, Vaughn V Smider, Peter G Schultz.   

Abstract

Bispecific antibodies were constructed using genetically encoded unnatural amino acids with orthogonal chemical reactivity. A two-step process afforded homogeneous products in excellent yield. Using this approach, we synthesized an anti-HER2/anti-CD3 bispecific antibody, which efficiently cross-linked HER2+ cells and CD3+ cells. In vitro effector-cell mediated cytotoxicity was observed at picomolar concentrations.

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Year:  2012        PMID： 22642368      PMCID： PMC4299457          DOI： 10.1021/ja303904e

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  32 in total

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  44 in total

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