BACKGROUND: Vital tissue provided by fresh frozen tissue banking is often required for genetic tumor profiling and tailored therapies. However, the potential patient benefits of fresh frozen tissue banking are currently limited to university hospitals. The objective of the present pilot study--the first one in the literature--was to evaluate whether fresh frozen tissue banking is feasible in a regional hospital without an integrated institute of pathology. METHODS: Patients with resectable breast and colon cancer were included in this prospective study. Both malignant and healthy tissue were sampled using isopentan-based snap-freezing 1 h after tumor resection and stored at -80 °C before transfer to the main tissue bank of a University institute of pathology. RESULTS: The initial costs to set up tissue banking were 35,662 US$. Furthermore, the running costs are 1,250 US$ yearly. During the first 13 months, 43 samples (nine samples of breast cancer and 34 samples of colon cancer) were collected from 41 patients. Based on the pathology reports, there was no interference with standard histopathologic analyses due to the sample collection. CONCLUSIONS: This is the first report in the literature providing evidence that tissue banking in a regional hospital without an integrated institute of pathology is feasible. The interesting findings of the present pilot study must be confirmed by larger investigations.
BACKGROUND: Vital tissue provided by fresh frozen tissue banking is often required for genetic tumor profiling and tailored therapies. However, the potential patient benefits of fresh frozen tissue banking are currently limited to university hospitals. The objective of the present pilot study--the first one in the literature--was to evaluate whether fresh frozen tissue banking is feasible in a regional hospital without an integrated institute of pathology. METHODS:Patients with resectable breast and colon cancer were included in this prospective study. Both malignant and healthy tissue were sampled using isopentan-based snap-freezing 1 h after tumor resection and stored at -80 °C before transfer to the main tissue bank of a University institute of pathology. RESULTS: The initial costs to set up tissue banking were 35,662 US$. Furthermore, the running costs are 1,250 US$ yearly. During the first 13 months, 43 samples (nine samples of breast cancer and 34 samples of colon cancer) were collected from 41 patients. Based on the pathology reports, there was no interference with standard histopathologic analyses due to the sample collection. CONCLUSIONS: This is the first report in the literature providing evidence that tissue banking in a regional hospital without an integrated institute of pathology is feasible. The interesting findings of the present pilot study must be confirmed by larger investigations.
Authors: Brian R Leyland-Jones; Christine B Ambrosone; John Bartlett; Matthew J C Ellis; Rebecca A Enos; Adekunle Raji; Michael R Pins; Jo Anne Zujewski; Stephen M Hewitt; John F Forbes; Mark Abramovitz; Sofia Braga; Fatima Cardoso; Nadia Harbeck; Carsten Denkert; Scott D Jewell Journal: J Clin Oncol Date: 2008-10-27 Impact factor: 44.544
Authors: E García-Granero; O Faiz; B Flor-Lorente; S García-Botello; P Esclápez; A Cervantes Journal: Colorectal Dis Date: 2010-03-02 Impact factor: 3.788
Authors: Leonard L Gunderson; John Milburn Jessup; Daniel J Sargent; Frederick L Greene; Andrew K Stewart Journal: J Clin Oncol Date: 2009-11-30 Impact factor: 44.544
Authors: S Mook; M K Schmidt; B Weigelt; B Kreike; I Eekhout; M J van de Vijver; A M Glas; A Floore; E J T Rutgers; L J van 't Veer Journal: Ann Oncol Date: 2009-10-13 Impact factor: 32.976
Authors: Dimitry S A Nuyten; Trevor Hastie; Jen-Tsan Ashley Chi; Howard Y Chang; Marc J van de Vijver Journal: Eur J Cancer Date: 2008-08-18 Impact factor: 9.162