Literature DB >> 2263458

A simple and sensitive method for determining transcription initiation site: identification of two transcription initiation sites in rat group II phospholipase A2 gene.

O Ohara1, J Ishizaki, T Nakano, H Arita, H Teraoka.   

Abstract

We developed a simple and sensitive method for assigning transcriptional initiation sites, and applied it to characterize the transcriptional unit of rat group II phospholipase A2 (PLA2) gene. Our method involves the primer extension reaction followed by detection of its products by hybridization. Using this method, we were able to map two transcriptional initiation sites on the nucleotide sequence of the core promoter region of PLA2 gene with one-base resolution without any difficulties.

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Year:  1990        PMID： 2263458      PMCID： PMC332761          DOI： 10.1093/nar/18.23.6997

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  34 in total

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2.  Structure of gene coding for rat group II phospholipase A2.

Authors:  M Komada; I Kudo; K Inoue
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6.  Genomic sequencing.

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7.  Improved hybridization assays employing tailed oligonucleotide probes: a direct comparison with 5'-end-labeled oligonucleotide probes and nick-translated plasmid probes.

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Authors:  L J Old
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  10 in total

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Authors: 
Journal:  Nucleic Acids Res       Date:  1991-02-25       Impact factor: 16.971

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Authors:  D Vial; L Arbibe; N Havet; C Dumarey; B Vargaftig; L Touqui
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3.  Phospholipase A2 isoforms are altered in chronic pancreatitis.

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Review 4.  Phospholipase A2-mediated hydrolysis of cardiac phospholipids: the use of molecular and transgenic techniques.

Authors:  L J De Windt; R S Reneman; G J Van der Vusse; M Van Bilsen
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Journal:  Gut       Date:  1999-10       Impact factor: 23.059

6.  Cell-specific regulation of type II phospholipase A2 expression in rat mesangial cells.

Authors:  M Konieczkowski; J R Sedor
Journal:  J Clin Invest       Date:  1993-11       Impact factor: 14.808

7.  Secretory phospholipase A2, group IIA is a novel serum amyloid A target gene: activation of smooth muscle cell expression by an interleukin-1 receptor-independent mechanism.

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9.  Transcriptional regulation of the rat type IIA phospholipase A2 gene by cAMP and interleukin-1beta in vascular smooth muscle cells: interplay of the CCAAT/enhancer binding protein (C/EBP), nuclear factor-kappaB and Ets transcription factors.

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10.  Neurofilament deficiency in quail caused by nonsense mutation in neurofilament-L gene.

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  10 in total

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