BACKGROUND: The prostate stem cell antigen (PSCA) is broadly overexpressed on the surface of prostate cancer cells. MATERIALS & METHODS: Anti-human PSCA monoclonal antibody (mAb 7F5) was bound to Fe(3)O(4)/Au (GoldMag) nanoparticles to serve as a PSCA-specific molecular MRI probe (mAb 7F5@GoldMag) for in vivo detection of prostate cancer cells. First, the efficacy of the antibody immobilization for the binding was assessed. Next, PC-3 (human prostate cancer cell line with PSCA overexpression) tumor-bearing mice were injected with mAb 7F5@GoldMag for MRI measurements while using mouse anti-human IgG bound to the particles (IgG@GoldMag) to serve as a nonspecific control. MRI examinations were conducted before and after injection of these probes at 6, 12 and 24 h; T2-weighted signal intensity within the tumors was measured. RESULTS: Targeted binding of the mAb 7F5@GoldMag probe to PC-3 tumors was verified with optical images and MRI; selective binding was not observed for the nonspecific IgG@GoldMag probe. CONCLUSION: MRI measurements suggest the promising efficacy of this new approach for targeted molecular imaging of prostate tumors.
BACKGROUND: The prostate stem cell antigen (PSCA) is broadly overexpressed on the surface of prostate cancer cells. MATERIALS & METHODS: Anti-humanPSCA monoclonal antibody (mAb 7F5) was bound to Fe(3)O(4)/Au (GoldMag) nanoparticles to serve as a PSCA-specific molecular MRI probe (mAb 7F5@GoldMag) for in vivo detection of prostate cancer cells. First, the efficacy of the antibody immobilization for the binding was assessed. Next, PC-3 (humanprostate cancer cell line with PSCA overexpression) tumor-bearing mice were injected with mAb 7F5@GoldMag for MRI measurements while using mouse anti-human IgG bound to the particles (IgG@GoldMag) to serve as a nonspecific control. MRI examinations were conducted before and after injection of these probes at 6, 12 and 24 h; T2-weighted signal intensity within the tumors was measured. RESULTS: Targeted binding of the mAb 7F5@GoldMag probe to PC-3tumors was verified with optical images and MRI; selective binding was not observed for the nonspecific IgG@GoldMag probe. CONCLUSION: MRI measurements suggest the promising efficacy of this new approach for targeted molecular imaging of prostate tumors.
Authors: Yang Guo; Zhuoli Zhang; Dong-Hyun Kim; Weiguo Li; Jodi Nicolai; Daniel Procissi; Yi Huan; Guohong Han; Reed A Omary; Andrew C Larson Journal: Int J Nanomedicine Date: 2013-09-06