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Literature DB >> 22617796

Polycistronic coexpression and nondenaturing purification of histone octamers.

Yoonjung Shim¹, Ming-Rui Duan, Xuejing Chen, Michael J Smerdon, Jung-Hyun Min.

Abstract

Histone octamers are the basic building blocks of chromatin and are platforms for diverse genetic mechanisms. We report a simple method for preparing recombinant histone octamers by overexpressing all four histones from a single polycistronic vector followed by standard chromatography under native conditions. This approach reduces the time needed for the octamer preparation to a single day and should be applicable to making a variety of unmodified and modified histone octamers.

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Year: 2012 PMID： 22617796 PMCID： PMC3412673 DOI： 10.1016/j.ab.2012.05.006

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

14 in total

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6. Reconstitution and Purification of Nucleosomes with Recombinant Histones and Purified DNA.

Authors: Ilana M Nodelman; Ashok Patel; Robert F Levendosky; Gregory D Bowman
Journal: Curr Protoc Mol Biol Date: 2020-12

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