Literature DB >> 22615135

Enhancement of the promiscuous aldolase and dehydration activities of 4-oxalocrotonate tautomerase by protein engineering.

Ellen Zandvoort1, Edzard M Geertsema, Wim J Quax, Gerrit J Poelarends.   

Abstract

Double play: The enzyme 4-oxalocrotonate tautomerase (4-OT) catalyzes not only the initial cross-coupling of acetaldehyde and benzaldehyde to yield 3-hydroxy-3-phenylpropanal, but also the subsequent dehydration of this aldol compound to yield cinnamaldehyde as the final product. Mechanism-inspired engineering provided an active site mutant (F50A) with strongly enhanced aldol condensation activity.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2012        PMID： 22615135     DOI： 10.1002/cbic.201200225

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  7 in total

Review 1.  Computational tools for rational protein engineering of aldolases.

Authors:  Michael Widmann; Jürgen Pleiss; Anne K Samland
Journal:  Comput Struct Biotechnol J       Date:  2012-11-13       Impact factor: 7.271

Review 2.  Engineering aldolases as biocatalysts.

Authors:  Claire L Windle; Marion Müller; Adam Nelson; Alan Berry
Journal:  Curr Opin Chem Biol       Date:  2014-01-04       Impact factor: 8.822

Review 3.  Building Bridges: Biocatalytic C-C-Bond Formation toward Multifunctional Products.

Authors:  Nina G Schmidt; Elisabeth Eger; Wolfgang Kroutil
Journal:  ACS Catal       Date:  2016-06-08       Impact factor: 13.084

4.  Engineering a Promiscuous Tautomerase into a More Efficient Aldolase for Self-Condensations of Linear Aliphatic Aldehydes.

Authors:  Mehran Rahimi; Jan-Ytzen van der Meer; Edzard M Geertsema; Gerrit J Poelarends
Journal:  Chembiochem       Date:  2017-05-30       Impact factor: 3.164

5.  Enantioselective Synthesis of Pharmaceutically Active γ-Aminobutyric Acids Using a Tailor-Made Artificial Michaelase in One-Pot Cascade Reactions.

Authors:  Lieuwe Biewenga; Thangavelu Saravanan; Andreas Kunzendorf; Jan-Ytzen van der Meer; Tjaard Pijning; Pieter G Tepper; Ronald van Merkerk; Simon J Charnock; Andy-Mark W H Thunnissen; Gerrit J Poelarends
Journal:  ACS Catal       Date:  2019-01-07       Impact factor: 13.084

6.  Demethionylation of Pro-1 variants of 4-oxalocrotonate tautomerase in Escherichia coli by co-expression with an engineered methionine aminopeptidase.

Authors:  Bert-Jan Baas; Ellen Zandvoort; Anna A Wasiel; Gerrit J Poelarends
Journal:  FEBS Open Bio       Date:  2014-07-09       Impact factor: 2.693

Review 7.  Enabling protein-hosted organocatalytic transformations.

Authors:  Alexander R Nödling; Nicolò Santi; Thomas L Williams; Yu-Hsuan Tsai; Louis Y P Luk
Journal:  RSC Adv       Date:  2020-04-23       Impact factor: 4.036
  7 in total

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