Literature DB >> 22613804

Impact of normalization method on experimental outcome using RT-qPCR in Staphylococcus aureus.

Lukas Valihrach1, Katerina Demnerova.   

Abstract

We performed a gene expression study using RT-qPCR in Staphylococcus aureus. The influence of normalization method was investigated. We confirmed that a recent standard, using more reference genes, was the best normalization strategy. The application of the most commonly used reference genes in 2011 (gyrB and 16S rRNA gene) failed.
Copyright © 2012 Elsevier B.V. All rights reserved.

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Year:  2012        PMID： 22613804     DOI： 10.1016/j.mimet.2012.05.008

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  25 in total

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Journal:  Antimicrob Agents Chemother       Date:  2016-05-23       Impact factor: 5.191

10.  VraCP regulates cell wall metabolism and antibiotic resistance in vancomycin-intermediate Staphylococcus aureus strain Mu50.

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Journal:  J Antimicrob Chemother       Date:  2021-06-18       Impact factor: 5.790
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