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Literature DB >> 22610994

Charge state coalescence during electrospray ionization improves peptide identification by tandem mass spectrometry.

Abstract

We report the effects of supercharging reagents dimethyl sulphoxide (DMSO) and m-nitrobenzyl alcohol (m-NBA) applied to untargeted peptide identification, with special emphasis on non-tryptic peptides. Peptides generated from a mixture of five standard proteins digested with trypsin, elastase, or pepsin were separated with nanoflow liquid chromatography using mobile phases modified with either 5% DMSO or 0.1%m-NBA. Eluting peptides were ionized by online electrospray and sequenced by both CID and ETD using data-dependent MS/MS. Statistically significant improvements in peptide identifications were observed with DMSO co-solvent. In order to understand this observation, we assessed the effects of supercharging reagents on the chromatographic separation and the electrospray quality. The increase in identifications was not due to supercharging, which was greater for the 0.1%m-NBA co-solvent and not observed for the 5.0% DMSO co-solvent. The improved MS/MS efficiency using the DMSO modified mobile phase appeared to result from charge state coalescence.

Entities: Chemical Disease Gene Species

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Year: 2012 PMID： 22610994 PMCID： PMC6345509 DOI： 10.1007/s13361-012-0404-0

Source DB: PubMed Journal: J Am Soc Mass Spectrom ISSN： 1044-0305 Impact factor: 3.109

33 in total

1. A study of ion suppression effects in electrospray ionization from mobile phase additives and solid-phase extracts.

Authors: Claude R Mallet; Ziling Lu; Jeff R Mazzeo
Journal: Rapid Commun Mass Spectrom Date: 2004 Impact factor: 2.419

2. The generating function of CID, ETD, and CID/ETD pairs of tandem mass spectra: applications to database search.

Authors: Sangtae Kim; Nikolai Mischerikow; Nuno Bandeira; J Daniel Navarro; Louis Wich; Shabaz Mohammed; Albert J R Heck; Pavel A Pevzner
Journal: Mol Cell Proteomics Date: 2010-09-09 Impact factor: 5.911

3. XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification.

Authors: Colin A Smith; Elizabeth J Want; Grace O'Maille; Ruben Abagyan; Gary Siuzdak
Journal: Anal Chem Date: 2006-02-01 Impact factor: 6.986

4. Supplemental activation method for high-efficiency electron-transfer dissociation of doubly protonated peptide precursors.

Authors: Danielle L Swaney; Graeme C McAlister; Matthew Wirtala; Jae C Schwartz; John E P Syka; Joshua J Coon
Journal: Anal Chem Date: 2007-01-15 Impact factor: 6.986

5. Improved electrospray ionization efficiency compensates for diminished chromatographic resolution and enables proteomics analysis of tyrosine signaling in embryonic stem cells.

Authors: Scott B Ficarro; Yi Zhang; Yu Lu; Ahmadali R Moghimi; Manor Askenazi; Elzbieta Hyatt; Eric D Smith; Leah Boyer; Thorsten M Schlaeger; C John Luckey; Jarrod A Marto
Journal: Anal Chem Date: 2009-05-01 Impact factor: 6.986

Review 6. Multidimensional LC separations in shotgun proteomics.

Authors: Akira Motoyama; John R Yates
Journal: Anal Chem Date: 2008-10-01 Impact factor: 6.986

7. Effects of mobile-phase additives, solution pH, ionization constant, and analyte concentration on the sensitivities and electrospray ionization mass spectra of nucleoside antiviral agents.

Authors: A M Kamel; P R Brown; B Munson
Journal: Anal Chem Date: 1999-12-15 Impact factor: 6.986

8. Real-time hydrogen/deuterium exchange kinetics via supercharged electrospray ionization tandem mass spectrometry.

Authors: Harry J Sterling; Evan R Williams
Journal: Anal Chem Date: 2010-10-13 Impact factor: 6.986

9. Studies of signal suppression in liquid chromatography-electrospray ionization mass spectrometry using volatile ion-pairing reagents.

Authors: S A Gustavsson; J Samskog; K E Markides; B Långström
Journal: J Chromatogr A Date: 2001-12-07 Impact factor: 4.759

10. Monte carlo simulation of macromolecular ionization by nanoelectrospray.

Authors: Christopher J Hogan; Pratim Biswas
Journal: J Am Soc Mass Spectrom Date: 2008-05-16 Impact factor: 3.109

16 in total

1. The use of chromium(III) to supercharge peptides by protonation at low basicity sites.

Authors: Changgeng Feng; Juliette J Commodore; Carolyn J Cassady
Journal: J Am Soc Mass Spectrom Date: 2014-11-14 Impact factor: 3.109

2. DMSO Assisted Electrospray Ionization for the Detection of Small Peptide Hormones in Urine by Dilute-and-Shoot-Liquid-Chromatography-High Resolution Mass Spectrometry.

Authors: Péter Judák; Janelle Grainger; Catrin Goebel; Peter Van Eenoo; Koen Deventer
Journal: J Am Soc Mass Spectrom Date: 2017-04-19 Impact factor: 3.109

Charge state coalescence during electrospray ionization improves peptide identification by tandem mass spectrometry.

1. A study of ion suppression effects in electrospray ionization from mobile phase additives and solid-phase extracts.

2. The generating function of CID, ETD, and CID/ETD pairs of tandem mass spectra: applications to database search.

3. XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification.

4. Supplemental activation method for high-efficiency electron-transfer dissociation of doubly protonated peptide precursors.

5. Improved electrospray ionization efficiency compensates for diminished chromatographic resolution and enables proteomics analysis of tyrosine signaling in embryonic stem cells.

Review 6. Multidimensional LC separations in shotgun proteomics.

7. Effects of mobile-phase additives, solution pH, ionization constant, and analyte concentration on the sensitivities and electrospray ionization mass spectra of nucleoside antiviral agents.

8. Real-time hydrogen/deuterium exchange kinetics via supercharged electrospray ionization tandem mass spectrometry.

9. Studies of signal suppression in liquid chromatography-electrospray ionization mass spectrometry using volatile ion-pairing reagents.

10. Monte carlo simulation of macromolecular ionization by nanoelectrospray.

1. The use of chromium(III) to supercharge peptides by protonation at low basicity sites.

2. DMSO Assisted Electrospray Ionization for the Detection of Small Peptide Hormones in Urine by Dilute-and-Shoot-Liquid-Chromatography-High Resolution Mass Spectrometry.

3. Expanding proteome coverage with orthogonal-specificity α-lytic proteases.

4. Label-free quantification in ion mobility-enhanced data-independent acquisition proteomics.

5. One-hour proteome analysis in yeast.

6. Accurate, Sensitive, and Precise Multiplexed Proteomics Using the Complement Reporter Ion Cluster.

7. What protein charging (and supercharging) reveal about the mechanism of electrospray ionization.

8. The Negative Mode Proteome with Activated Ion Negative Electron Transfer Dissociation (AI-NETD).

9. Enhancing Sensitivity of Liquid Chromatography-Mass Spectrometry of Peptides and Proteins Using Supercharging Agents.

10. DMSO enhances electrospray response, boosting sensitivity of proteomic experiments.