Literature DB >> 22610439

One-step sequence- and ligation-independent cloning as a rapid and versatile cloning method for functional genomics studies.

Jae-Yeon Jeong1, Hyung-Soon Yim, Ji-Young Ryu, Hyun Sook Lee, Jung-Hyun Lee, Dong-Seung Seen, Sung Gyun Kang.   

Abstract

We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.

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Year:  2012        PMID： 22610439      PMCID： PMC3416421          DOI： 10.1128/AEM.00844-12

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  15 in total

1.  GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes.

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Journal:  Methods Enzymol       Date:  2000       Impact factor: 1.600

2.  Functional cloning vectors for use in directional cDNA cloning using cohesive ends produced with T4 DNA polymerase.

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Journal:  Gene       Date:  1992-03-15       Impact factor: 3.688

3.  Ligation-independent cloning of PCR products (LIC-PCR).

Authors:  C Aslanidis; P J de Jong
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

4.  Enzyme free cloning for high throughput gene cloning and expression.

Authors:  Rob N de Jong; Mark A Daniëls; Rob Kaptein; Gert E Folkers
Journal:  J Struct Funct Genomics       Date:  2007-02-13

5.  Disruption of a sugar transporter gene cluster in a hyperthermophilic archaeon using a host-marker system based on antibiotic resistance.

Authors:  Rie Matsumi; Kenji Manabe; Toshiaki Fukui; Haruyuki Atomi; Tadayuki Imanaka
Journal:  J Bacteriol       Date:  2007-01-26       Impact factor: 3.490

Review 6.  Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda.

Authors:  A Kuzminov
Journal:  Microbiol Mol Biol Rev       Date:  1999-12       Impact factor: 11.056

7.  A new logic for DNA engineering using recombination in Escherichia coli.

Authors:  Y Zhang; F Buchholz; J P Muyrers; A F Stewart
Journal:  Nat Genet       Date:  1998-10       Impact factor: 38.330

8.  The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism.

Authors:  Hyun Sook Lee; Sung Gyun Kang; Seung Seob Bae; Jae Kyu Lim; Yona Cho; Yun Jae Kim; Jeong Ho Jeon; Sun-Shin Cha; Kae Kyoung Kwon; Hyung-Tae Kim; Cheol-Joo Park; Hee-Wook Lee; Seung Il Kim; Jongsik Chun; Rita R Colwell; Sang-Jin Kim; Jung-Hyun Lee
Journal:  J Bacteriol       Date:  2008-09-12       Impact factor: 3.490

9.  Genetics Techniques for Thermococcus kodakarensis.

Authors:  Travis H Hileman; Thomas J Santangelo
Journal:  Front Microbiol       Date:  2012-06-08       Impact factor: 5.640

10.  Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase.

Authors:  Michael D Hamilton; Anthony A Nuara; Don B Gammon; R Mark Buller; David H Evans
Journal:  Nucleic Acids Res       Date:  2006-12-07       Impact factor: 16.971
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  140 in total

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Journal:  Plant Cell       Date:  2019-04-08       Impact factor: 11.277

2.  Alternative linker histone permits fast paced nuclear divisions in early Drosophila embryo.

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Journal:  Nucleic Acids Res       Date:  2020-09-18       Impact factor: 16.971

3.  The Legionella longbeachae Icm/Dot substrate SidC selectively binds phosphatidylinositol 4-phosphate with nanomolar affinity and promotes pathogen vacuole-endoplasmic reticulum interactions.

Authors:  Stephanie Dolinsky; Ina Haneburger; Adam Cichy; Mandy Hannemann; Aymelt Itzen; Hubert Hilbi
Journal:  Infect Immun       Date:  2014-07-14       Impact factor: 3.441

4.  Homologous recombinatorial cloning without the creation of single-stranded ends: exonuclease and ligation-independent cloning (ELIC).

Authors:  Essi V Koskela; Alexander D Frey
Journal:  Mol Biotechnol       Date:  2015-03       Impact factor: 2.695

5.  Genetic redundancy is prevalent within the 6.7 Mb Sinorhizobium meliloti genome.

Authors:  George C diCenzo; Turlough M Finan
Journal:  Mol Genet Genomics       Date:  2015-02-01       Impact factor: 3.291

6.  Molecular interactions between tubulin tails and glutamylases reveal determinants of glutamylation patterns.

Authors:  Kathiresan Natarajan; Sudarshan Gadadhar; Judith Souphron; Maria M Magiera; Carsten Janke
Journal:  EMBO Rep       Date:  2017-05-08       Impact factor: 8.807

7.  PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis.

Authors:  Hyun Kim; Ae Kyung Park; Jun Hyuck Lee; Seung Chul Shin; Hyun Park; Han Woo Kim
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2018-05-31       Impact factor: 1.056

8.  Purine Permease-Type Benzylisoquinoline Alkaloid Transporters in Opium Poppy.

Authors:  Mehran Dastmalchi; Limei Chang; Rongji Chen; Lisa Yu; Xue Chen; Jillian M Hagel; Peter J Facchini
Journal:  Plant Physiol       Date:  2019-08-29       Impact factor: 8.340

9.  Direct Electron Transfer between the frhAGB-Encoded Hydrogenase and Thioredoxin Reductase in the Nonmethanogenic Archaeon Thermococcus onnurineus NA1.

Authors:  Hae-Chang Jung; Jae Kyu Lim; Tae-Jun Yang; Sung Gyun Kang; Hyun Sook Lee
Journal:  Appl Environ Microbiol       Date:  2020-03-02       Impact factor: 4.792

10.  Compartmentalization of phosphatidylinositol 4,5-bisphosphate metabolism into plasma membrane liquid-ordered/raft domains.

Authors:  Jongyun Myeong; Cheon-Gyu Park; Byung-Chang Suh; Bertil Hille
Journal:  Proc Natl Acad Sci U S A       Date:  2021-03-02       Impact factor: 11.205
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