Literature DB >> 22608734

Transformation of Clostridium thermocellum by electroporation.

Daniel G Olson1, Lee R Lynd.   

Abstract

In this work, we provide detailed instructions for transformation of Clostridium thermocellum by electroporation. In addition, we describe two schemes for genetic modification: allelic replacement-where the gene of interest is replaced by an antibiotic marker and markerless gene deletion-where the gene of interest is removed and the selective markers are recycled. The markerless gene deletion technique can also be used for insertion of genes onto the C. thermocellum chromosome.
Copyright © 2012 Elsevier Inc. All rights reserved.

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Year:  2012        PMID： 22608734     DOI： 10.1016/B978-0-12-415931-0.00017-3

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  49 in total

1.  The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is necessary for ethanol production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum.

Authors:  Jonathan Lo; Tianyong Zheng; Shuen Hon; Daniel G Olson; Lee R Lynd
Journal:  J Bacteriol       Date:  2015-02-09       Impact factor: 3.490

2.  Deletion of the Clostridium thermocellum recA gene reveals that it is required for thermophilic plasmid replication but not plasmid integration at homologous DNA sequences.

Authors:  Joseph Groom; Daehwan Chung; Sun-Ki Kim; Adam Guss; Janet Westpheling
Journal:  J Ind Microbiol Biotechnol       Date:  2018-05-28       Impact factor: 3.346

3.  Determining the roles of the three alcohol dehydrogenases (AdhA, AdhB and AdhE) in Thermoanaerobacter ethanolicus during ethanol formation.

Authors:  Jilai Zhou; Xiongjun Shao; Daniel G Olson; Sean Jean-Loup Murphy; Liang Tian; Lee R Lynd
Journal:  J Ind Microbiol Biotechnol       Date:  2017-01-11       Impact factor: 3.346

4.  LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313.

Authors:  Charlotte M Wilson; Dawn M Klingeman; Caleb Schlachter; Mustafa H Syed; Chia-Wei Wu; Adam M Guss; Steven D Brown
Journal:  Appl Environ Microbiol       Date:  2017-02-15       Impact factor: 4.792

5.  CO2-fixing one-carbon metabolism in a cellulose-degrading bacterium Clostridium thermocellum.

Authors:  Wei Xiong; Paul P Lin; Lauren Magnusson; Lisa Warner; James C Liao; Pin-Ching Maness; Katherine J Chou
Journal:  Proc Natl Acad Sci U S A       Date:  2016-10-28       Impact factor: 11.205

6.  Cofactor Specificity of the Bifunctional Alcohol and Aldehyde Dehydrogenase (AdhE) in Wild-Type and Mutant Clostridium thermocellum and Thermoanaerobacterium saccharolyticum.

Authors:  Tianyong Zheng; Daniel G Olson; Liang Tian; Yannick J Bomble; Michael E Himmel; Jonathan Lo; Shuen Hon; A Joe Shaw; Johannes P van Dijken; Lee R Lynd
Journal:  J Bacteriol       Date:  2015-05-26       Impact factor: 3.490

7.  Ferredoxin:NAD+ Oxidoreductase of Thermoanaerobacterium saccharolyticum and Its Role in Ethanol Formation.

Authors:  Liang Tian; Jonathan Lo; Xiongjun Shao; Tianyong Zheng; Daniel G Olson; Lee R Lynd
Journal:  Appl Environ Microbiol       Date:  2016-11-21       Impact factor: 4.792

8.  Role of the CipA scaffoldin protein in cellulose solubilization, as determined by targeted gene deletion and complementation in Clostridium thermocellum.

Authors:  Daniel G Olson; Richard J Giannone; Robert L Hettich; Lee R Lynd
Journal:  J Bacteriol       Date:  2012-11-30       Impact factor: 3.490

9.  Expression of adhA from different organisms in Clostridium thermocellum.

Authors:  Tianyong Zheng; Jingxuan Cui; Hye Ri Bae; Lee R Lynd; Daniel G Olson
Journal:  Biotechnol Biofuels       Date:  2017-11-30       Impact factor: 6.040

10.  Identifying promoters for gene expression in Clostridium thermocellum.

Authors:  Daniel G Olson; Marybeth Maloney; Anthony A Lanahan; Shuen Hon; Loren J Hauser; Lee R Lynd
Journal:  Metab Eng Commun       Date:  2015-03-30
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