Literature DB >> 22591122

Verification of the role of MlrC in microcystin biodegradation by studies using a heterologously expressed enzyme.

Dariusz Dziga1, Marcin Wasylewski, Adam Szetela, Oliwia Bocheńska, Benedykt Wladyka.   

Abstract

The MlrC protein from Sphingomonas ACM-3962 strain was heterologously expressed in Escherichia coli strain BL21(DE3) and purified to investigate participation of this enzyme in the biodegradation of two microcystin variants. In contrast with previous reports, our results indicated that MlrC cleaves linear microcystins, thus shedding new light on the role of MlrC enzyme in microcystin biodegradation.

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Year:  2012        PMID： 22591122     DOI： 10.1021/tx300174e

Source DB:  PubMed          Journal:  Chem Res Toxicol        ISSN： 0893-228X            Impact factor:   3.739


  6 in total

1.  Bioreactor study employing bacteria with enhanced activity toward cyanobacterial toxins microcystins.

Authors:  Dariusz Dziga; Magdalena Lisznianska; Benedykt Wladyka
Journal:  Toxins (Basel)       Date:  2014-08-13       Impact factor: 4.546

2.  Isolation of a Novel Microcystin-Degrading Bacterium and the Evolutionary Origin of mlr Gene Cluster.

Authors:  Lian Qin; Xiaoxing Zhang; Xiaoguo Chen; Ke Wang; Yitian Shen; Dan Li
Journal:  Toxins (Basel)       Date:  2019-05-13       Impact factor: 4.546

Review 3.  A Mini Review on Microcystins and Bacterial Degradation.

Authors:  Isaac Yaw Massey; Fei Yang
Journal:  Toxins (Basel)       Date:  2020-04-21       Impact factor: 4.546

4.  Characterization of Enzymatic Activity of MlrB and MlrC Proteins Involved in Bacterial Degradation of Cyanotoxins Microcystins.

Authors:  Dariusz Dziga; Gabriela Zielinska; Benedykt Wladyka; Oliwia Bochenska; Anna Maksylewicz; Wojciech Strzalka; Jussi Meriluoto
Journal:  Toxins (Basel)       Date:  2016-03-16       Impact factor: 4.546

5.  Microbial Degradation of Amino Acid-Containing Compounds Using the Microcystin-Degrading Bacterial Strain B-9.

Authors:  Haiyan Jin; Yoshiko Hiraoka; Yurie Okuma; Elisabete Hiromi Hashimoto; Miki Kurita; Andrea Roxanne J Anas; Hitoshi Uemura; Kiyomi Tsuji; Ken-Ichi Harada
Journal:  Mar Drugs       Date:  2018-02-06       Impact factor: 5.118

6.  Cloning and Expression of Genes for Biodegrading Nodularin by Sphingopyxis sp. USTB-05.

Authors:  Qianqian Xu; Hongfei Ma; Jinhui Fan; Hai Yan; Haiyang Zhang; Chunhua Yin; Xiaolu Liu; Yang Liu; Huasheng Wang
Journal:  Toxins (Basel)       Date:  2019-09-20       Impact factor: 4.546
  6 in total

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