BACKGROUND: CLAUSINE B, A CARBAZOLE ALKALOID ISOLATED FROM THE STEM BARK OF CLAUSENA EXCAVATA, WAS INVESTIGATED FOR ITS ANTIPROLIFERATIVE ACTIVITIES AGAINST FIVE HUMAN CANCER CELL LINES: HepG2 (hepatic cancer), MCF-7 (hormone-dependent breast cancer), MDA-MB-231 (non-hormone-dependent breast cancer), HeLa (cervical cancer), and CAOV3 (ovarian cancer). METHODS: Chang liver (normal cells) was used as a control. The effect of clausine-B was measured using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. RESULTS: Clausine-B was found to be active (IC(50)<30 μg/mL) against four of the cancer cell lines tested. The IC(50) values for these four lines were: 21.50 μg/mL (MDA-MB-231), 22.90 g/ml (HeLa), 27.00 μg/mL (CAOV3) and 28.94 μg/mL (HepG2). Clausine-B inhibited the MCF-7 cancer cell line at 52.90 μg/mL, and no IC(50) value was obtained against Chang liver. CONCLUSION: It is possible that the phenolic group in clausine-B responsible for the antiproliferative activities found in this study.
BACKGROUND:CLAUSINE B, A CARBAZOLE ALKALOID ISOLATED FROM THE STEM BARK OF CLAUSENA EXCAVATA, WAS INVESTIGATED FOR ITS ANTIPROLIFERATIVE ACTIVITIES AGAINST FIVE HUMANCANCER CELL LINES: HepG2 (hepatic cancer), MCF-7 (hormone-dependent breast cancer), MDA-MB-231 (non-hormone-dependent breast cancer), HeLa (cervical cancer), and CAOV3 (ovarian cancer). METHODS: Chang liver (normal cells) was used as a control. The effect of clausine-B was measured using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. RESULTS:Clausine-B was found to be active (IC(50)<30 μg/mL) against four of the cancer cell lines tested. The IC(50) values for these four lines were: 21.50 μg/mL (MDA-MB-231), 22.90 g/ml (HeLa), 27.00 μg/mL (CAOV3) and 28.94 μg/mL (HepG2). Clausine-B inhibited the MCF-7 cancer cell line at 52.90 μg/mL, and no IC(50) value was obtained against Chang liver. CONCLUSION: It is possible that the phenolic group in clausine-B responsible for the antiproliferative activities found in this study.
Entities:
Keywords:
Clausena excavata; Clausine-B; cell survival; ethnopharmacology; medical sciences
Authors: Shaymaa Fadhel Abbas Albaayit; Rukesh Maharjan; Rasedee Abdullah; Mohd Hezmee Mohd Noor Journal: Biomed Res Int Date: 2021-02-23 Impact factor: 3.411