Literature DB >> 22576748

Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5.

Hajra Basit1, K Shivaji Sharma, Angéline Van der Heyden, Chantal Gondran, Cécile Breyton, Pascal Dumy, Françoise M Winnik, Pierre Labbé.   

Abstract

Biotinylated amphipol was used to entrap FhuA (an E. coli outer membrane protein) and immobilize the FhuA-amphipol complex on streptavidin surfaces. Using this assembly, we have successfully devised surface-based assays for studying the recognition of FhuA by pb5 (a bacteriophage T5 protein) and determination of the affinity constant.

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Year:  2012        PMID： 22576748     DOI： 10.1039/c2cc31107k

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  7 in total

1.  Assessing the conformational changes of pb5, the receptor-binding protein of phage T5, upon binding to its Escherichia coli receptor FhuA.

Authors:  Cécile Breyton; Ali Flayhan; Frank Gabel; Mathilde Lethier; Grégory Durand; Pascale Boulanger; Mohamed Chami; Christine Ebel
Journal:  J Biol Chem       Date:  2013-09-06       Impact factor: 5.157

2.  Amphipol-mediated screening of molecular orthoses specific for membrane protein targets.

Authors:  Yann Ferrandez; Manuela Dezi; Mickael Bosco; Agathe Urvoas; Marie Valerio-Lepiniec; Christel Le Bon; Fabrice Giusti; Isabelle Broutin; Grégory Durand; Ange Polidori; Jean-Luc Popot; Martin Picard; Philippe Minard
Journal:  J Membr Biol       Date:  2014-08-03       Impact factor: 1.843

Review 3.  Labeling and functionalizing amphipols for biological applications.

Authors:  Christel Le Bon; Jean-Luc Popot; Fabrice Giusti
Journal:  J Membr Biol       Date:  2014-04-03       Impact factor: 1.843

Review 4.  Functionalized amphipols: a versatile toolbox suitable for applications of membrane proteins in synthetic biology.

Authors:  Eduardo Antonio Della Pia; Randi Westh Hansen; Manuela Zoonens; Karen L Martinez
Journal:  J Membr Biol       Date:  2014-04-13       Impact factor: 1.843

Review 5.  Amphipols for each season.

Authors:  Manuela Zoonens; Jean-Luc Popot
Journal:  J Membr Biol       Date:  2014-06-27       Impact factor: 1.843

6.  Synthesis of an oligonucleotide-derivatized amphipol and its use to trap and immobilize membrane proteins.

Authors:  Christel Le Bon; Eduardo Antonio Della Pia; Fabrice Giusti; Noémie Lloret; Manuela Zoonens; Karen L Martinez; Jean-Luc Popot
Journal:  Nucleic Acids Res       Date:  2014-04-17       Impact factor: 16.971

7.  Stabilization of a Membrane-Associated Amyloid-β Oligomer for Its Validation in Alzheimer's Disease.

Authors:  Montserrat Serra-Batiste; James Tolchard; Fabrice Giusti; Manuela Zoonens; Natàlia Carulla
Journal:  Front Mol Biosci       Date:  2018-04-19
  7 in total

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