Peter Takacs1, Yanping Zhang, Keith Candiotti, Sindy Jaramillo, Carlos A Medina. 1. Department of Obstetrics and Gynecology, Division of Female Pelvic Medicine and Reconstructive Surgery, University of Miami, Miller School of Medicine, PO Box 016960, Miami, FL 33010, USA. ptakacs@med.miami.edu
Abstract
INTRODUCTION AND HYPOTHESIS: The objective was to measure the effects of peroxisome proliferator-activated receptor delta (PPAR-delta) agonist and zinc sulfate (ZS) on vaginal smooth muscle cell (SMC) proliferation, tropoelastin production, and collagen production. METHODS: SMC cultures were performed from vaginal wall biopsies and were incubated with ZS and PPAR-delta agonist GW501516 (GW). Cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Supernatants and cell lysates were collected. Tropoelastin production was measured by Fastin Elastin Assay and collagen was measured with Sircol Collagen Assay. RESULTS: SMC proliferation was similar with 20 μM ZS or 10 nM GW compared to control. Tropoelastin production was significantly increased by 20 μM ZS and by 10 nM GW. Cell culture surface deposited elastin production was significantly increased by 20 μM ZS and by the combination of 20 μM ZS with 10 nM GW, and collagen production was significantly increased by 10 nM GW and by the combination of 20 μM ZS with 10 nM GW. CONCLUSIONS: PPAR-delta agonist and ZS increases vaginal SMC tropoelastin and collagen production.
INTRODUCTION AND HYPOTHESIS: The objective was to measure the effects of peroxisome proliferator-activated receptor delta (PPAR-delta) agonist and zinc sulfate (ZS) on vaginal smooth muscle cell (SMC) proliferation, tropoelastin production, and collagen production. METHODS: SMC cultures were performed from vaginal wall biopsies and were incubated with ZS and PPAR-delta agonist GW501516 (GW). Cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Supernatants and cell lysates were collected. Tropoelastin production was measured by Fastin Elastin Assay and collagen was measured with Sircol Collagen Assay. RESULTS: SMC proliferation was similar with 20 μM ZS or 10 nM GW compared to control. Tropoelastin production was significantly increased by 20 μM ZS and by 10 nM GW. Cell culture surface deposited elastin production was significantly increased by 20 μM ZS and by the combination of 20 μM ZS with 10 nM GW, and collagen production was significantly increased by 10 nM GW and by the combination of 20 μM ZS with 10 nM GW. CONCLUSIONS:PPAR-delta agonist and ZS increases vaginal SMC tropoelastin and collagen production.
Authors: Marcos L Sznaidman; Curt D Haffner; Patrick R Maloney; Adam Fivush; Esther Chao; Donna Goreham; Michael L Sierra; Christelle LeGrumelec; H Eric Xu; Valerie G Montana; Millard H Lambert; Timothy M Willson; William R Oliver; Daniel D Sternbach Journal: Bioorg Med Chem Lett Date: 2003-05-05 Impact factor: 2.940
Authors: Anett Csikós; Bence Kozma; Edina Baranyai; Ida Miklós; Kindra Larson; Róbert Póka; Peter Takacs Journal: BMC Womens Health Date: 2021-02-11 Impact factor: 2.809