| Literature DB >> 22568620 |
Rung-Yi Lai1, Siyu Huang, Michael K Fenwick, Amrita Hazra, Yang Zhang, Kanagalaghatta Rajashankar, Benjamin Philmus, Cynthia Kinsland, Jennie Mansell Sanders, Steven E Ealick, Tadhg P Begley.
Abstract
In Saccharomyces cerevisiae , thiamin pyrimidine is formed from histidine and pyridoxal phosphate (PLP). The origin of all of the pyrimidine atoms has been previously determined using labeling studies and suggests that the pyrimidine is formed using remarkable chemistry that is without chemical or biochemical precedent. Here we report the overexpression of the closely related Candida albicans pyrimidine synthase (THI5p) and the reconstitution and preliminary characterization of the enzymatic activity. A structure of the C. albicans THI5p shows PLP bound at the active site via an imine with Lys62 and His66 in close proximity to the PLP. Our data suggest that His66 of the THI5 protein is the histidine source for pyrimidine formation and that the pyrimidine synthase is a single-turnover enzyme.Entities:
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Year: 2012 PMID： 22568620 PMCID： PMC3415583 DOI： 10.1021/ja302474a
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419