Literature DB >> 22567191

PNA HyBeacons for analysis of human mutations related to statin-induced myopathy.

Nittaya Gale1, Petr Kocalka, Charlotte Mardle, Tom Brown.   

Abstract

Aminoalkyl and alkyne-tagged PNA HyBeacons have been synthesized, labeled with fluorescein via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA HyBeacons gave much better mismatch/mutant discrimination than conventional DNA HyBeacons but smaller fluorescence changes on melting.

Entities:  

Keywords:  HyBeacon; PNA; SNPs; fluorescence melting; genetic analysis; statin-induced myopathy

Mesh：

Substances：

Year:  2011        PMID： 22567191      PMCID： PMC3324338          DOI： 10.4161/adna.18179

Source DB:  PubMed          Journal:  Artif DNA PNA XNA        ISSN： 1949-095X


  17 in total

1.  Thermodynamics of sequence-specific binding of PNA to DNA.

Authors:  T Ratilainen; A Holmén; E Tuite; P E Nielsen; B Nordén
Journal:  Biochemistry       Date:  2000-07-04       Impact factor: 3.162

2.  Synthesis of 5- and 6-hydroxymethylfluorescein phosphoramidites.

Authors:  M Adamczyk; C M Chan; J R Fino; P G Mattingly
Journal:  J Org Chem       Date:  2000-01-28       Impact factor: 4.354

3.  Detection of PCR products using self-probing amplicons and fluorescence.

Authors:  D Whitcombe; J Theaker; S P Guy; T Brown; S Little
Journal:  Nat Biotechnol       Date:  1999-08       Impact factor: 54.908

4.  HyBeacon probes: a new tool for DNA sequence detection and allele discrimination.

Authors:  D J French; C L Archard; T Brown; D G McDowell
Journal:  Mol Cell Probes       Date:  2001-12       Impact factor: 2.365

5.  Fluorescence based strategies for genetic analysis.

Authors:  Rohan T Ranasinghe; Tom Brown
Journal:  Chem Commun (Camb)       Date:  2005-09-30       Impact factor: 6.222

Review 6.  High resolution melting applications for clinical laboratory medicine.

Authors:  Maria Erali; Karl V Voelkerding; Carl T Wittwer
Journal:  Exp Mol Pathol       Date:  2008-04-13       Impact factor: 3.362

7.  Molecular beacons: probes that fluoresce upon hybridization.

Authors:  S Tyagi; F R Kramer
Journal:  Nat Biotechnol       Date:  1996-03       Impact factor: 54.908

8.  Real time quantitative PCR.

Authors:  C A Heid; J Stevens; K J Livak; P M Williams
Journal:  Genome Res       Date:  1996-10       Impact factor: 9.043

9.  Peptide nucleic acid probes with charged photocleavable mass markers: Towards PNA-based MALDI-TOF MS genetic analysis.

Authors:  Rachel J Ball; Philip S Green; Nittaya Gale; G John Langley; Tom Brown
Journal:  Artif DNA PNA XNA       Date:  2010-07

Review 10.  Click chemistry with DNA.

Authors:  Afaf H El-Sagheer; Tom Brown
Journal:  Chem Soc Rev       Date:  2010-02-09       Impact factor: 54.564
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