| Literature DB >> 22554463 |
Kota Sathish1, Rajan Sriraman, B Mohana Subramanian, N Hanumantha Rao, Balaji Kasa, Jagan Donikeni, M Lakshmi Narasu, V A Srinivasan.
Abstract
Coccidiosis is a disease caused by intracellular parasites belonging to the genus Eimeria. In the present study, we transiently expressed two coccidial antigens EtMIC1 and EtMIC2 as poly histidine-tagged fusion proteins in tobacco. We have evaluated the protective efficacy of plant expressed EtMIC1 as monovalent and as well as bi-valent formulation where EtMIC1 and EtMIC2 were used in combination. The protective efficacy of these formulations was evaluated using homologous challenge in chickens. We observed better serum antibody response, weight gain and reduced oocyst shedding in birds immunized with EtMIC1 and EtMIC2 as bivalent formulation compared to monovalent formulation. However, IFN-γ response was not significant in birds immunized with EtMIC1 compared to the birds immunized with EtMIC2. Our results indicate the potential use of these antigens as vaccine candidates.Entities:
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Year: 2012 PMID： 22554463 DOI： 10.1016/j.vaccine.2012.04.076
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641