Literature DB >> 2254285

Purification and partial characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Streptomyces clavuligerus.

S E Jensen1, A Wong, M J Rollins, D W Westlake.   

Abstract

delta-(L-alpha-Aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS) was purified from Streptomyces clavuligerus by a combination of salt precipitation, ultrafiltration, and anion-exchange chromatography. The final purified material gave two protein bands with molecular weights of 283,000 and 32,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Electrophoresis in nondenaturing gels gave a single protein band with an estimated molecular weight of 560,000. These results suggest that ACVS is a multimer composed of nonidentical subunits.

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Year:  1990        PMID： 2254285      PMCID： PMC210854          DOI： 10.1128/jb.172.12.7269-7271.1990

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  10 in total

1.  STARCH-GEL ELECTROPHORESIS--APPLICATION TO THE CLASSIFICATION OF PITUITARY PROTEINS AND POLYPEPTIDES.

Authors:  K A FERGUSON
Journal:  Metabolism       Date:  1964-10       Impact factor: 8.694

2.  Gramicidin S biosynthesis operon containing the structural genes grsA and grsB has an open reading frame encoding a protein homologous to fatty acid thioesterases.

Authors:  J Krätzschmar; M Krause; M A Marahiel
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

3.  Determination of low-molecular-weight thiols using monobromobimane fluorescent labeling and high-performance liquid chromatography.

Authors:  R C Fahey; G L Newton
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

Review 4.  Peptide antibiotics, beta-lactams, and related compounds.

Authors:  H Kleinkauf; H von Döhren
Journal:  Crit Rev Biotechnol       Date:  1988       Impact factor: 8.429

5.  Systems for polyacrylamide gel electrophoresis.

Authors:  P J Blackshear
Journal:  Methods Enzymol       Date:  1984       Impact factor: 1.600

6.  A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids.

Authors:  D Wessel; U I Flügge
Journal:  Anal Biochem       Date:  1984-04       Impact factor: 3.365

7.  delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Aspergillus nidulans. The first enzyme in penicillin biosynthesis is a multifunctional peptide synthetase.

Authors:  H van Liempt; H von Döhren; H Kleinkauf
Journal:  J Biol Chem       Date:  1989-03-05       Impact factor: 5.157

8.  Cell-free synthesis of delta-(L-alpha-aminoadipyl)-L-cysteine, the first intermediate of penicillin and cephalosporin biosynthesis.

Authors:  G Banko; S Wolfe; A L Demain
Journal:  Biochem Biophys Res Commun       Date:  1986-05-29       Impact factor: 3.575

9.  Sulfhydryl groups related to the catalytic activity of gramicidin S synthetase 1 of Bacillus brevis.

Authors:  M Kanda; K Hori; T Kurotsu; S Miura; Y Yamada; Y Saito
Journal:  J Biochem       Date:  1981-09       Impact factor: 3.387

10.  Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II.

Authors:  H Gushima; S Yasuda; E Soeda; M Yokota; M Kondo; A Kimura
Journal:  Nucleic Acids Res       Date:  1984-12-21       Impact factor: 16.971
  10 in total
  13 in total

1.  Nucleotide sequence analysis reveals linked N-acetyl hydrolase, thioesterase, transport, and regulatory genes encoded by the bialaphos biosynthetic gene cluster of Streptomyces hygroscopicus.

Authors:  A Raibaud; M Zalacain; T G Holt; R Tizard; C J Thompson
Journal:  J Bacteriol       Date:  1991-07       Impact factor: 3.490

Review 2.  δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS): discovery and perspectives.

Authors:  Kapil Tahlan; Marcus A Moore; Susan E Jensen
Journal:  J Ind Microbiol Biotechnol       Date:  2016-10-20       Impact factor: 3.346

3.  Purification and characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Penicillium chrysogenum.

Authors:  H B Theilgaard; K N Kristiansen; C M Henriksen; J Nielsen
Journal:  Biochem J       Date:  1997-10-01       Impact factor: 3.857

Review 4.  Molecular regulation of beta-lactam biosynthesis in filamentous fungi.

Authors:  A A Brakhage
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

5.  Substrate specificity of L-delta-(alpha-aminoadipoyl)-L-cysteinyl-D-valine synthetase from Cephalosporium acremonium: demonstration of the structure of several unnatural tripeptide products.

Authors:  J E Baldwin; C Y Shiau; M F Byford; C J Schofield
Journal:  Biochem J       Date:  1994-07-15       Impact factor: 3.857

6.  Production of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine by entrapped ACV-synthetase from Streptomyces clavuligerus.

Authors:  T A Kadima; S E Jensen; M A Pickard
Journal:  J Ind Microbiol       Date:  1995-01

7.  Production kinetics and stability properties of delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Streptomyces clavuligerus.

Authors:  T A Kadima; S E Jensen; M A Pickard
Journal:  J Ind Microbiol       Date:  1993-01

8.  Localization of the lysine epsilon-aminotransferase (lat) and delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (pcbAB) genes from Streptomyces clavuligerus and production of lysine epsilon-aminotransferase activity in Escherichia coli.

Authors:  M B Tobin; S Kovacevic; K Madduri; J A Hoskins; P L Skatrud; L C Vining; C Stuttard; J R Miller
Journal:  J Bacteriol       Date:  1991-10       Impact factor: 3.490

9.  Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis.

Authors:  T K Wu; R W Busby; T A Houston; D B McIlwaine; L A Egan; C A Townsend
Journal:  J Bacteriol       Date:  1995-07       Impact factor: 3.490

Review 10.  Clusters of genes for the biosynthesis of antibiotics: regulatory genes and overproduction of pharmaceuticals.

Authors:  J F Martin
Journal:  J Ind Microbiol       Date:  1992 Feb-Mar
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