Determination of total flavonoids content in fresh Ginkgo biloba leaf with different colors using near infrared spectroscopy.

Total flavonoids content is often considered an important quality index of Ginkgo biloba leaf. The feasibility of using near infrared (NIR) spectra at the wavelength range of 10,000-4000cm(-1) for rapid and nondestructive determination of total flavonoids content in G. biloba leaf was investigated. 120 fresh G. biloba leaves in different colors (green, green-yellowish and yellow) were used to spectra acquisition and total flavonoids determination. Partial least squares (PLS), interval partial least squares (iPLS) and synergy interval partial least squares (SiPLS) were used to develop calibration models for total flavonoids content in two colors leaves (green-yellowish and yellow) and three colors leaves (green, green-yellowish and yellow), respectively. The level of total flavonoids content for green, green-yellowish and yellow leaves was in an increasing order. Two characteristic wavelength regions (5840-6090cm(-1) and 6620-6880cm(-1)), which corresponded to the absorptions of two aromatic rings in basic flavonoid structure, were selected by SiPLS. The optimal SiPLS model for total flavonoids content in the two colors leaves (r(2)=0.82, RMSEP=2.62mg g(-1)) had better performance than PLS and iPLS models. It could be concluded that NIR spectroscopy has significant potential in the nondestructive determination of total flavonoids content in fresh G. biloba leaf.