Literature DB >> 22518861

Recombinase polymerase amplification assay for rapid detection of Francisella tularensis.

Milena Euler1, Yongjie Wang, Peter Otto, Herbert Tomaso, Raquel Escudero, Pedro Anda, Frank T Hufert, Manfred Weidmann.   

Abstract

Several real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real-time PCR but reduced the assay time to 10 min. The rapid RPA method has great application potential for field use or point-of-care diagnostics.

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Year:  2012        PMID： 22518861      PMCID： PMC3405570          DOI： 10.1128/JCM.06504-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  29 in total

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Review 4.  Miniaturized isothermal nucleic acid amplification, a review.

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Review 5.  Strategies for highly sensitive biomarker detection by Rolling Circle Amplification of signals from nucleic acid composed sensors.

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Review 6.  Diagnostic procedures in tularaemia with special focus on molecular and immunological techniques.

Authors:  W D Splettstoesser; H Tomaso; S Al Dahouk; H Neubauer; P Schuff-Werner
Journal:  J Vet Med B Infect Dis Vet Public Health       Date:  2005-08

7.  Rapid detection of human pathogenic orthobunyaviruses.

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8.  Helicase-dependent isothermal DNA amplification.

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9.  Description of Francisella hispaniensis sp. nov., isolated from human blood, reclassification of Francisella novicida (Larson et al. 1955) Olsufiev et al. 1959 as Francisella tularensis subsp. novicida comb. nov. and emended description of the genus Francisella.

Authors:  Birgit Huber; Raquel Escudero; Hans-Jürgen Busse; Erik Seibold; Holger C Scholz; Pedro Anda; Peter Kämpfer; Wolf D Splettstoesser
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Review 10.  Syndromic surveillance and bioterrorism-related epidemics.

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Journal:  Emerg Infect Dis       Date:  2003-10       Impact factor: 6.883
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  50 in total

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2.  Early detection of dengue virus by use of reverse transcription-recombinase polymerase amplification.

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Journal:  J Clin Microbiol       Date:  2015-01-07       Impact factor: 5.948

3.  Development of a panel of recombinase polymerase amplification assays for detection of biothreat agents.

Authors:  Milena Euler; Yongjie Wang; Doris Heidenreich; Pranav Patel; Oliver Strohmeier; Sydney Hakenberg; Matthias Niedrig; Frank T Hufert; Manfred Weidmann
Journal:  J Clin Microbiol       Date:  2013-01-23       Impact factor: 5.948

4.  Rapid genome detection of Schmallenberg virus and bovine viral diarrhea virus by use of isothermal amplification methods and high-speed real-time reverse transcriptase PCR.

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5.  Multiplex isothermal solid-phase recombinase polymerase amplification for the specific and fast DNA-based detection of three bacterial pathogens.

Authors:  Sebastian Kersting; Valentina Rausch; Frank F Bier; Markus von Nickisch-Rosenegk
Journal:  Mikrochim Acta       Date:  2014-02-18       Impact factor: 5.833

6.  Molecular neuroimaging of post-injury plasticity.

Authors:  Yan Jouroukhin; Bareng A S Nonyane; Assaf A Gilad; Galit Pelled
Journal:  J Mol Neurosci       Date:  2014-06-10       Impact factor: 3.444

7.  Rapid Detection of Cyprinid Herpesvirus 3 in Latently Infected Koi by Recombinase Polymerase Amplification.

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Journal:  J Aquat Anim Health       Date:  2016-09       Impact factor: 1.625

8.  Rapid detection of highly pathogenic porcine reproductive and respiratory syndrome virus by a fluorescent probe-based isothermal recombinase polymerase amplification assay.

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9.  Development of a panel of recombinase polymerase amplification assays for detection of common bacterial urinary tract infection pathogens.

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10.  A Novel Molecular Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care.

Authors:  Alejandro Castellanos-Gonzalez; Omar A Saldarriaga; Lilian Tartaglino; Rosana Gacek; Elissa Temple; Hayley Sparks; Peter C Melby; Bruno L Travi
Journal:  Am J Trop Med Hyg       Date:  2015-08-03       Impact factor: 2.345
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