BACKGROUND: Hepatitis B virus (HBV) is still one of the serious infectious risks for the blood transfusion safety in China. One plausible reason is the emergence of the variants in the major antigenic alpha determinant within the major hydrophilic region (MHR) of hepatitis B surface antigen (HBsAg), which have been assumed to evade the immune surveillance and pose a challenge to the disease diagnosis. It is well documented that some commercial ELISA kits could detect the wild-type but not the mutant viruses. The high prevalence of HBV in China also impaired the application of nucleic acid testing (NAT) in the improvement of blood security. Molecular epidemiological study of HBsAg variations in China is still limited. This study was designed to identify the prevalence of mutations in the HBsAg in voluntary blood donors in Nanjing, China. METHODS: A total of 20,326 blood units were enrolled in this study, 39 donors were positive for HBV S gene in the nested-PCR. Mutations in the major hydrophilic region (MHR; aa 99-169) were identified by direct sequencing of S region. RESULTS: Among of 20,326 blood units in the Red Cross Transfusion Center of Nanjing from October 2008 to April 2009, 296 samples (1.46%, 296/20,326) were HBsAg positive in the 2 successive rounds of the ELISA test. In these HBsAg positive units, HBV S gene could be successfully amplified from 39 donors (13.18%, 39/296) in the nested-PCR. Sequence analysis revealed that 32 strains (82.1%, 32/39) belong to genotype B, 7 strains (17.9%, 7/39) to genotype C. Besides well known G145R, widely dispersed variations in the MHR of S region, were observed in 20 samples of all the strains sequenced. CONCLUSIONS: HBV/B and HBV/C are dominant in Nanjing, China. The mutations in the MHR of HBsAg associated with disease diagnosis are common.
BACKGROUND:Hepatitis B virus (HBV) is still one of the serious infectious risks for the blood transfusion safety in China. One plausible reason is the emergence of the variants in the major antigenic alpha determinant within the major hydrophilic region (MHR) of hepatitis B surface antigen (HBsAg), which have been assumed to evade the immune surveillance and pose a challenge to the disease diagnosis. It is well documented that some commercial ELISA kits could detect the wild-type but not the mutant viruses. The high prevalence of HBV in China also impaired the application of nucleic acid testing (NAT) in the improvement of blood security. Molecular epidemiological study of HBsAg variations in China is still limited. This study was designed to identify the prevalence of mutations in the HBsAg in voluntary blood donors in Nanjing, China. METHODS: A total of 20,326 blood units were enrolled in this study, 39 donors were positive for HBV S gene in the nested-PCR. Mutations in the major hydrophilic region (MHR; aa 99-169) were identified by direct sequencing of S region. RESULTS: Among of 20,326 blood units in the Red Cross Transfusion Center of Nanjing from October 2008 to April 2009, 296 samples (1.46%, 296/20,326) were HBsAg positive in the 2 successive rounds of the ELISA test. In these HBsAg positive units, HBV S gene could be successfully amplified from 39 donors (13.18%, 39/296) in the nested-PCR. Sequence analysis revealed that 32 strains (82.1%, 32/39) belong to genotype B, 7 strains (17.9%, 7/39) to genotype C. Besides well known G145R, widely dispersed variations in the MHR of S region, were observed in 20 samples of all the strains sequenced. CONCLUSIONS:HBV/B and HBV/C are dominant in Nanjing, China. The mutations in the MHR of HBsAg associated with disease diagnosis are common.
Authors: Susan L Stramer; Shimian Zou; Edward P Notari; Gregory A Foster; David E Krysztof; Fatemeh Musavi; Roger Y Dodd Journal: Transfusion Date: 2011-08-02 Impact factor: 3.157
Authors: Varsha Thakur; Syed N Kazim; Rajkumar C Guptan; Seyed E Hasnain; Angeline Bartholomeusz; V Malhotra; Shiv K Sarin Journal: J Med Virol Date: 2005-05 Impact factor: 2.327
Authors: S Seddigh-Tonekaboni; W L Lim; B Young; J L Hou; J Waters; K X Luo; H C Thomas; P Karayiannis Journal: J Viral Hepat Date: 2001-03 Impact factor: 3.728
Authors: Nicola Coppola; Lorenzo Onorato; Carmine Minichini; Giovanni Di Caprio; Mario Starace; Caterina Sagnelli; Evangelista Sagnelli Journal: World J Hepatol Date: 2015-11-28
Authors: X Y Wang; T J Harrison; X He; Q Y Chen; G J Li; M H Liu; H Li; J Y Yang; Z L Fang Journal: Epidemiol Infect Date: 2015-04-23 Impact factor: 4.434
Authors: Haimanti Bhattacharya; Debdutta Bhattacharya; S R Ghosal; Subarna Roy; A P Sugunan Journal: Indian J Med Res Date: 2015-05 Impact factor: 2.375