PURPOSE: These experiments assessed the ability of spectral-domain optical coherence tomography (SD-OCT) to accurately represent the structural organization of the adult zebrafish retina and reveal the dynamic morphologic changes during either light-induced damage and regeneration of photoreceptors or ouabain-induced inner retinal damage. METHODS: Retinas of control dark-adapted adult albino zebrafish were compared with retinas subjected to 24 hours of constant intense light and recovered for up to 8 weeks or ouabain-damaged retinas that recovered for up to 3 weeks. Images were captured and the measurements of retinal morphology were made by SD-OCT, and then compared with those obtained by histology of the same eyes. RESULTS: Measurements between SD-OCT and histology were very similar for the undamaged, damaged, and regenerating retinas. Axial measurements of SD-OCT also revealed vitreal morphology that was not readily visualized by histology. CONCLUSIONS: SD-OCT accurately represented retinal lamination and photoreceptor loss and recovery during light-induced damage and subsequent regeneration. SD-OCT was less accurate at detecting the inner nuclear layer in ouabain-damaged retinas, but accurately detected the undamaged outer nuclear layer. Thus, SD-OCT provides a noninvasive and quantitative method to assess the morphology and the extent of damage and repair in the zebrafish retina.
PURPOSE: These experiments assessed the ability of spectral-domain optical coherence tomography (SD-OCT) to accurately represent the structural organization of the adult zebrafish retina and reveal the dynamic morphologic changes during either light-induced damage and regeneration of photoreceptors or ouabain-induced inner retinal damage. METHODS: Retinas of control dark-adapted adult albino zebrafish were compared with retinas subjected to 24 hours of constant intense light and recovered for up to 8 weeks or ouabain-damaged retinas that recovered for up to 3 weeks. Images were captured and the measurements of retinal morphology were made by SD-OCT, and then compared with those obtained by histology of the same eyes. RESULTS: Measurements between SD-OCT and histology were very similar for the undamaged, damaged, and regenerating retinas. Axial measurements of SD-OCT also revealed vitreal morphology that was not readily visualized by histology. CONCLUSIONS:SD-OCT accurately represented retinal lamination and photoreceptor loss and recovery during light-induced damage and subsequent regeneration. SD-OCT was less accurate at detecting the inner nuclear layer in ouabain-damaged retinas, but accurately detected the undamaged outer nuclear layer. Thus, SD-OCT provides a noninvasive and quantitative method to assess the morphology and the extent of damage and repair in the zebrafish retina.
Authors: Maciej Wojtkowski; Rainer Leitgeb; Andrzej Kowalczyk; Tomasz Bajraszewski; Adolf F Fercher Journal: J Biomed Opt Date: 2002-07 Impact factor: 3.170
Authors: Ki Hean Kim; Mehron Puoris'haag; Gopi N Maguluri; Yumiko Umino; Karen Cusato; Robert B Barlow; Johannes F de Boer Journal: J Vis Date: 2008-01-24 Impact factor: 2.240
Authors: Bhavna J Antony; Michael D Abràmoff; Matthew M Harper; Woojin Jeong; Elliott H Sohn; Young H Kwon; Randy Kardon; Mona K Garvin Journal: Biomed Opt Express Date: 2013-11-01 Impact factor: 3.732
Authors: Rose M DiCicco; Brent A Bell; Charles Kaul; Joe G Hollyfield; Bela Anand-Apte; Brian D Perkins; Yuankai K Tao; Alex Yuan Journal: Invest Ophthalmol Vis Sci Date: 2014-09-09 Impact factor: 4.799
Authors: David Troilo; Earl L Smith; Debora L Nickla; Regan Ashby; Andrei V Tkatchenko; Lisa A Ostrin; Timothy J Gawne; Machelle T Pardue; Jody A Summers; Chea-Su Kee; Falk Schroedl; Siegfried Wahl; Lyndon Jones Journal: Invest Ophthalmol Vis Sci Date: 2019-02-28 Impact factor: 4.799