Literature DB >> 22493334

Multiplex PCR for rapid and improved diagnosis of bloodstream infections in liver transplant recipients.

Peter-Michael Rath1, Fuat Saner, Andreas Paul, Nils Lehmann, Eike Steinmann, Jan Buer, Joerg Steinmann.   

Abstract

This prospective study evaluated the utility of the SeptiFast (SF) test in detecting 25 clinically important pathogens in 225 blood samples from 170 intensive care unit (ICU) patients with suspected sepsis after liver transplantation (LTX) or after other major abdominal surgery (non-LTX). SF yielded a significantly higher positivity rate in the LTX group (52.3%) than in the non-LTX group (30.5%; P = 0.0009). SF may be a powerful tool for the early diagnosis of bloodstream infections in LTX patients.

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Year:  2012        PMID： 22493334      PMCID： PMC3372100          DOI： 10.1128/JCM.00745-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

1.  Minimizing the workup of blood culture contaminants: implementation and evaluation of a laboratory-based algorithm.

Authors:  S S Richter; S E Beekmann; J L Croco; D J Diekema; F P Koontz; M A Pfaller; G V Doern
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

2.  Epidemiology and outcome of invasive fungal infections in solid organ transplant recipients.

Authors:  D Neofytos; J A Fishman; D Horn; E Anaissie; C-H Chang; A Olyaei; M Pfaller; W J Steinbach; K M Webster; K A Marr
Journal:  Transpl Infect Dis       Date:  2010-01-25       Impact factor: 2.228

3.  Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia.

Authors:  F Lamoth; K Jaton; G Prod'hom; L Senn; J Bille; T Calandra; O Marchetti
Journal:  J Clin Microbiol       Date:  2010-08-18       Impact factor: 5.948

4.  Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies.

Authors:  Nicasio Mancini; Daniela Clerici; Roberta Diotti; Mario Perotti; Nadia Ghidoli; Donata De Marco; Beatrice Pizzorno; Thomas Emrich; Roberto Burioni; Fabio Ciceri; Massimo Clementi
Journal:  J Med Microbiol       Date:  2008-05       Impact factor: 2.472

Review 5.  2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference.

Authors:  Mitchell M Levy; Mitchell P Fink; John C Marshall; Edward Abraham; Derek Angus; Deborah Cook; Jonathan Cohen; Steven M Opal; Jean-Louis Vincent; Graham Ramsay
Journal:  Crit Care Med       Date:  2003-04       Impact factor: 7.598

6.  Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis.

Authors:  Barbara Lucignano; Stefania Ranno; Oliver Liesenfeld; Beatrice Pizzorno; Lorenza Putignani; Paola Bernaschi; Donato Menichella
Journal:  J Clin Microbiol       Date:  2011-04-06       Impact factor: 5.948

7.  Utility of a commercially available multiplex real-time PCR assay to detect bacterial and fungal pathogens in febrile neutropenia.

Authors:  Marie von Lilienfeld-Toal; Lutz E Lehmann; Ansgar D Raadts; Corinna Hahn-Ast; Katjana S Orlopp; Günter Marklein; Ingvill Purr; Gordon Cook; Andreas Hoeft; Axel Glasmacher; Frank Stüber
Journal:  J Clin Microbiol       Date:  2009-07-01       Impact factor: 5.948

8.  Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis.

Authors:  H Westh; G Lisby; F Breysse; B Böddinghaus; M Chomarat; V Gant; A Goglio; A Raglio; H Schuster; F Stuber; H Wissing; A Hoeft
Journal:  Clin Microbiol Infect       Date:  2009-04-21       Impact factor: 8.067

9.  Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group.

Authors:  Ben De Pauw; Thomas J Walsh; J Peter Donnelly; David A Stevens; John E Edwards; Thierry Calandra; Peter G Pappas; Johan Maertens; Olivier Lortholary; Carol A Kauffman; David W Denning; Thomas F Patterson; Georg Maschmeyer; Jacques Bille; William E Dismukes; Raoul Herbrecht; William W Hope; Christopher C Kibbler; Bart Jan Kullberg; Kieren A Marr; Patricia Muñoz; Frank C Odds; John R Perfect; Angela Restrepo; Markus Ruhnke; Brahm H Segal; Jack D Sobel; Tania C Sorrell; Claudio Viscoli; John R Wingard; Theoklis Zaoutis; John E Bennett
Journal:  Clin Infect Dis       Date:  2008-06-15       Impact factor: 9.079

10.  Incidence and significance of Aspergillus cultures following liver and kidney transplantation.

Authors:  R S Brown; J R Lake; B A Katzman; N L Ascher; K A Somberg; J C Emond; J P Roberts
Journal:  Transplantation       Date:  1996-02-27       Impact factor: 4.939
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  11 in total

1.  Non-culture-based methods to diagnose bloodstream infection: Does it work?

Authors:  Miha Skvarc; David Stubljar; Petra Rogina; Achim J Kaasch
Journal:  Eur J Microbiol Immunol (Bp)       Date:  2013-06-05

Review 2.  Accuracy of LightCycler(®) SeptiFast for the detection and identification of pathogens in the blood of patients with suspected sepsis: a systematic review and meta-analysis.

Authors:  Paul Dark; Bronagh Blackwood; Simon Gates; Danny McAuley; Gavin D Perkins; Ronan McMullan; Claire Wilson; Daniel Graham; Kate Timms; Geoffrey Warhurst
Journal:  Intensive Care Med       Date:  2014-11-22       Impact factor: 17.440

3.  Molecular diagnosis of sepsis: New aspects and recent developments.

Authors:  O Liesenfeld; L Lehman; K-P Hunfeld; G Kost
Journal:  Eur J Microbiol Immunol (Bp)       Date:  2014-03-14

Review 4.  Emerging Technologies for Molecular Diagnosis of Sepsis.

Authors:  Mridu Sinha; Julietta Jupe; Hannah Mack; Todd P Coleman; Shelley M Lawrence; Stephanie I Fraley
Journal:  Clin Microbiol Rev       Date:  2018-02-28       Impact factor: 26.132

5.  New approaches to sepsis: molecular diagnostics and biomarkers.

Authors:  Konrad Reinhart; Michael Bauer; Niels C Riedemann; Christiane S Hartog
Journal:  Clin Microbiol Rev       Date:  2012-10       Impact factor: 26.132

6.  Detection of Aspergillus fumigatus in Blood Samples from Critically Ill Patients in Intensive Care Units by Use of the SeptiFast Assay.

Authors:  Joerg Steinmann; Jan Buer; Peter-Michael Rath
Journal:  J Clin Microbiol       Date:  2016-05-04       Impact factor: 5.948

7.  Rapid diagnosis of bloodstream infections with PCR followed by mass spectrometry.

Authors:  Elena Jordana-Lluch; Heather E Carolan; Montserrat Giménez; Rangarajan Sampath; David J Ecker; M Dolores Quesada; Josep M Mòdol; Fernando Arméstar; Lawrence B Blyn; Lendell L Cummins; Vicente Ausina; Elisa Martró
Journal:  PLoS One       Date:  2013-04-23       Impact factor: 3.240

8.  Bacteraemia and fungaemia in cystic fibrosis patients with febrile pulmonary exacerbation: a prospective observational study.

Authors:  Joerg Grosse-Onnebrink; Florian Stehling; Eva Tschiedel; Margarete Olivier; Uwe Mellies; Rene Schmidt; Jan Buer; Peter-Micheal Rath; Joerg Steinmann
Journal:  BMC Pulm Med       Date:  2017-06-29       Impact factor: 3.317

9.  Implementation of short incubation MALDI-TOF MS identification from positive blood cultures in routine diagnostics and effects on empiric antimicrobial therapy.

Authors:  Robin Köck; Jörg Wüllenweber; Dagmar Horn; Christian Lanckohr; Karsten Becker; Evgeny A Idelevich
Journal:  Antimicrob Resist Infect Control       Date:  2017-01-14       Impact factor: 4.887

Review 10.  Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis - a systemic review and meta-analysis.

Authors:  Shy-Shin Chang; Wen-Han Hsieh; Ting-Shou Liu; Si-Huei Lee; Chih-Hung Wang; Hao-Chang Chou; Yee Hui Yeo; Ching-Ping Tseng; Chien-Chang Lee
Journal:  PLoS One       Date:  2013-05-29       Impact factor: 3.240
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