BACKGROUND: Alterations in glycosylation have long been associated with the development of cancer. In the case of primary hepatocellular carcinoma (HCC), one alteration that has often been associated is increased amounts of fucose attached to the N-glycans of serum proteins secreted by the liver. METHODS: In an effort to determine the origin of this increased fucosylation, we have conducted N-linked glycan analysis of HCC tissue, the surrounding nontumor tissue, and compared this to tissue from a nondiseased adult liver. RESULTS: Surprisingly, no difference in the level of fucosylation was observed from the three donor groups, suggesting that the increased levels of fucosylation observed in serum of those with HCC is not the result of increased synthesis of fucosylated proteins in the cancer tissue. On the other hand, increased levels of a tetra-antennary glycan were observed in the HCC tissue as compared with the surrounding tissue or to the nondiseased livers. CONCLUSIONS: This represents, to our knowledge, one of the first reports associating increased levels of branching with the development of HCC. IMPACT: The identification of increased levels of tetra-antennary glycan on liver tumor tissue, as opposed to adjacent or nondiseased tissue may lead to improved detection of HCC.
BACKGROUND: Alterations in glycosylation have long been associated with the development of cancer. In the case of primary hepatocellular carcinoma (HCC), one alteration that has often been associated is increased amounts of fucose attached to the N-glycans of serum proteins secreted by the liver. METHODS: In an effort to determine the origin of this increased fucosylation, we have conducted N-linked glycan analysis of HCC tissue, the surrounding nontumor tissue, and compared this to tissue from a nondiseased adult liver. RESULTS: Surprisingly, no difference in the level of fucosylation was observed from the three donor groups, suggesting that the increased levels of fucosylation observed in serum of those with HCC is not the result of increased synthesis of fucosylated proteins in the cancer tissue. On the other hand, increased levels of a tetra-antennary glycan were observed in the HCC tissue as compared with the surrounding tissue or to the nondiseased livers. CONCLUSIONS: This represents, to our knowledge, one of the first reports associating increased levels of branching with the development of HCC. IMPACT: The identification of increased levels of tetra-antennary glycan on liver tumor tissue, as opposed to adjacent or nondiseased tissue may lead to improved detection of HCC.
Authors: Y Ito; E Miyoshi; M Sakon; T Takeda; K Noda; M Tsujimoto; S Ito; H Honda; F Takemura; K Wakasa; M Monden; N Matsuura; N Taniguchi Journal: Int J Cancer Date: 2001-03-01 Impact factor: 7.396
Authors: Louise Royle; Taj S Mattu; Edmund Hart; James I Langridge; Anthony H Merry; Neil Murphy; David J Harvey; Raymond A Dwek; Pauline M Rudd Journal: Anal Biochem Date: 2002-05-01 Impact factor: 3.365
Authors: Steedman A Sarbah; Terry Gramlich; Abraham Younoszai; Pat Osmack; Marlene Goormastic; Leonard Grosso; James N Cooper; Adrian Di Bisceglie; Russell Seneca; Zobair M Younossi Journal: Dig Dis Sci Date: 2004-05 Impact factor: 3.199
Authors: K Noda; E Miyoshi; T Kitada; S Nakahara; C-X Gao; K Honke; Y Shiratori; H Moriwaki; Y Sasaki; A Kasahara; M Hori; N Hayashi; N Taniguchi Journal: Tumour Biol Date: 2002 Jul-Aug
Authors: L Renee Ruhaak; Sandra L Taylor; Carol Stroble; Uyen Thao Nguyen; Evan A Parker; Ting Song; Carlito B Lebrilla; William N Rom; Harvey Pass; Kyoungmi Kim; Karen Kelly; Suzanne Miyamoto Journal: J Proteome Res Date: 2015-09-30 Impact factor: 4.466